| Literature DB >> 15081616 |
Masashi Harazaki1, Yohei Kawai, Li Su, Yoko Hamazaki, Tatsutoshi Nakahata, Nagahiro Minato, Masakazu Hattori.
Abstract
SPA-1 is involved in the regulation of T cell activation in response to antigens through the control of Rap1 GTPase signaling. In this study, the subcellular localization of SPA-1 in the T cells was examined by using anti-SPA-1 antibody and GFP-SPA-1. While SPA-1 was detected diffusely at the surface cortical region in the floating unpolarized T cells, it was concentrated at the matrix-adhesion region with dense actin-cytoskeleton. Upon interaction with specific antigen-presenting cells, SPA-1 was highly concentrated at the immunological synapse closely co-localizing with actin. By yeast two-hybrid system, SPA-1 was shown to interact with an actin-bundling protein alpha-actinin, and it was indicated that SPA-1 co-localized with alpha-actinin at the immunological synapse. The results have suggested that SPA-1 in the T cells is selectively recruited to the immunological synapse with dense actin-cytoskeletal reorganization and keeps restraining the levels of Rap1GTP at the local TCR-signaling complex for the T cell activation.Entities:
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Year: 2004 PMID： 15081616 DOI： 10.1016/j.imlet.2004.01.004
Source DB: PubMed Journal: Immunol Lett ISSN： 0165-2478 Impact factor: 3.685