Literature DB >> 15081278

Purification and pH stability characterization of a chymotrypsin inhibitor from Schizolobium parahyba seeds.

Rozeni C L Teles1, Elizabeth M T de Souza, Leonardo de A Calderon, Sonia M de Freitas.   

Abstract

Schizolobium parahyba chymotrypsin inhibitor (SPCI) was completely purified as a single polypeptide chain with two disulfide bonds, by TCA precipitation and ion exchange chromatography. This purification method is faster and more efficient than that previously reported: SPCI is stable from pH 2 to 12 at 25 degrees C, and is highly specific for chymotrypsin at pH 7-12. It weakly inhibits elastase and has no significant inhibitory effect against trypsin and alpha-amylase. SPCI is a thermostable protein and resists thermolysin digestion up to 70 degrees C.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15081278     DOI： 10.1016/j.phytochem.2004.02.002

Source DB:  PubMed          Journal:  Phytochemistry        ISSN： 0031-9422            Impact factor:   4.072


  4 in total

1.  pH dependence thermal stability of a chymotrypsin inhibitor from Schizolobium parahyba seeds.

Authors:  Rozeni C L Teles; Leonardo de A Calderon; Francisco J Medrano; João A R G Barbosa; Beatriz G Guimarães; Marcelo M Santoro; Sonia M de Freitas
Journal:  Biophys J       Date:  2005-03-11       Impact factor: 4.033

2.  Crystallization and preliminary crystallographic studies of Schizolobium parahyba chymotrypsin inhibitor (SPCI) at 1.8 A resolution.

Authors:  Rozeni Chagas Lima Teles; Gisele Ferreira Esteves; Marcus Aurélio Miranda Araújo; Carlos Bloch; João Alexandre Ribeiro Gonçalves Barbosa; Sonia Maria de Freitas
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-10-24

3.  The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle.

Authors:  Sheyla V Lucena; Fabíola P Rufino; Gioconda Emanuella Diniz de Dantas Moura; Luciana M A Rabêlo; Norberto K V Monteiro; André T Ferreira; Jonas E Aguilar Perales; Adriana F Uchôa; Giselle Z Justo; Caio F R de Oliveira; Ludovico Migliolo; Helena Bonciani Nader; Elizeu A Santos; Adeliana S Oliveira
Journal:  3 Biotech       Date:  2021-12-15       Impact factor: 2.406

4.  Purification, crystallization and preliminary crystallographic studies of SPCI-chymotrypsin complex at 2.8 A resolution.

Authors:  Adelson Joel da Silva; Rozeni Chagas Lima Teles; Gisele Ferreira Esteves; Camila Ramos dos Santos; João Alexandre Ribeiro Gonçalves Barbosa; Sonia Maria de Freitas
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-09-30
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.