| Literature DB >> 15081031 |
Maciej Adamczyk1, James R Fino, Phillip G Mattingly, Jeffrey A Moore, You Pan.
Abstract
N(10)-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N(10) or 9-position carboxamide. Upon binding to folate binding protein the light output of the N(10) derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous chemiluminescent assay for folic acid.Entities:
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Year: 2004 PMID： 15081031 DOI： 10.1016/j.bmcl.2004.01.105
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823