Literature DB >> 15081006

Preparation of highly active alpha-chymotrypsin for catalysis in organic media.

Ipsita Roy1, Munishwar N Gupta.   

Abstract

A simple one step process for the preparation of free alpha-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.

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Year:  2004        PMID： 15081006     DOI： 10.1016/j.bmcl.2004.02.026

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  6 in total

1.  Dual bioimprinting of Thermomyces lanuginosus lipase for synthesis of biodiesel.

Authors:  Joyeeta Mukherjee; Munishwar Nath Gupta
Journal:  Biotechnol Rep (Amst)       Date:  2016-03-02

Review 2.  Increasing importance of protein flexibility in designing biocatalytic processes.

Authors:  Joyeeta Mukherjee; Munishwar Nath Gupta
Journal:  Biotechnol Rep (Amst)       Date:  2015-04-02

3.  Solid state fluorescence of proteins in high throughput mode and its applications.

Authors:  Saurabh Gautam; Munishwar N Gupta
Journal:  F1000Res       Date:  2013-03-11

4.  Alpha chymotrypsin coated clusters of Fe3O4 nanoparticles for biocatalysis in low water media.

Authors:  Joyeeta Mukherjee; Munishwar N Gupta
Journal:  Chem Cent J       Date:  2012-11-08       Impact factor: 4.215

5.  Optimising biocatalyst design for obtaining high transesterification activity by alpha-chymotrypsin in non-aqueous media.

Authors:  Kusum Solanki; Munishwar Nath Gupta
Journal:  Chem Cent J       Date:  2008-02-12       Impact factor: 4.215

6.  The effect of ultrasonic pre-treatment on the catalytic activity of lipases in aqueous and non-aqueous media.

Authors:  Shweta Shah; Munishwar Nath Gupta
Journal:  Chem Cent J       Date:  2008-01-30       Impact factor: 4.215
  6 in total

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