AIMS: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. METHODS AND RESULTS: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10 degrees C than at 20 degrees C. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. CONCLUSIONS: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. SIGNIFICANCE AND IMPACT OF THE STUDY: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications.
AIMS: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. METHODS AND RESULTS: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10 degrees C than at 20 degrees C. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. CONCLUSIONS: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. SIGNIFICANCE AND IMPACT OF THE STUDY: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications.
Authors: Matthew J Neave; Claire Streten-Joyce; Chris J Glasby; Keith A McGuinness; David L Parry; Karen S Gibb Journal: Microb Ecol Date: 2011-10-27 Impact factor: 4.552
Authors: Consolazione Caruso; Carmen Rizzo; Santina Mangano; Annarita Poli; Paola Di Donato; Ilaria Finore; Barbara Nicolaus; Gaetano Di Marco; Luigi Michaud; Angelina Lo Giudice Journal: Appl Environ Microbiol Date: 2018-01-31 Impact factor: 4.792
Authors: Carol Mancuso Nichols; Sandrine Garon Lardière; John P Bowman; Peter D Nichols; John A E Gibson; Jean Guézennec Journal: Microb Ecol Date: 2005-07-29 Impact factor: 4.552
Authors: Consolazione Caruso; Carmen Rizzo; Santina Mangano; Annarita Poli; Paola Di Donato; Barbara Nicolaus; Gaetano Di Marco; Luigi Michaud; Angelina Lo Giudice Journal: Environ Sci Pollut Res Int Date: 2017-12-01 Impact factor: 4.223