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Literature DB >> 15073005

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.

Abstract

UNLABELLED: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design. AVAILABILITY: Open-source and freely available from http://perlprimer.sourceforge.net.

Entities: Chemical

Mesh：

Substances：
DNA Primers
Sulfites

Year: 2004 PMID： 15073005 DOI： 10.1093/bioinformatics/bth254

Source DB: PubMed Journal: Bioinformatics ISSN： 1367-4803 Impact factor: 6.937

Keyword Cloud
Cited

210 in total

1. Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages.

Authors: P F Almaida-Pagán; C De Santis; O L Rubio-Mejía; D R Tocher
Journal: J Comp Physiol B Date: 2014-11-15 Impact factor: 2.200

2. Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus.

Authors: Benjamin Schusser; Antje Reuter; Alexander von der Malsburg; Nicola Penski; Steffen Weigend; Bernd Kaspers; Peter Staeheli; Sonja Härtle
Journal: J Virol Date: 2011-06-01 Impact factor: 5.103

3. Active transcription and essential role of RNA polymerase II at the centromere during mitosis.

Authors: F Lyn Chan; Owen J Marshall; Richard Saffery; Bo Won Kim; Elizabeth Earle; K H Andy Choo; Lee H Wong
Journal: Proc Natl Acad Sci U S A Date: 2012-01-20 Impact factor: 11.205

4. Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4.

Authors: Mohammad Mahfuz Chowdhury; Hiroshi Kimura; Teruo Fujii; Yasuyuki Sakai
Journal: Biomicrofluidics Date: 2012-03-07 Impact factor: 2.800

5. Normalizing RT-qPCR data: are we getting the right answers? An appraisal of normalization approaches and internal reference genes from a case study in the finfish Lates calcarifer.

Authors: Christian De Santis; Carolyn Smith-Keune; Dean R Jerry
Journal: Mar Biotechnol (NY) Date: 2010-03-23 Impact factor: 3.619

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.

1. Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages.

2. Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus.

3. Active transcription and essential role of RNA polymerase II at the centromere during mitosis.

4. Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4.

5. Normalizing RT-qPCR data: are we getting the right answers? An appraisal of normalization approaches and internal reference genes from a case study in the finfish Lates calcarifer.

6. Variability in melanoma metalloproteinase expression profiling.

7. Aligned electrospun scaffolds and elastogenic factors for vascular cell-mediated elastic matrix assembly.

8. Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana.

9. Sulforaphane causes a major epigenetic repression of myostatin in porcine satellite cells.

10. Suppression of retinal neovascularization by erythropoietin siRNA in a mouse model of proliferative retinopathy.