OBJECTIVE: To reveal gap junction connexin 43 (Cx43) in rabbit with early myocardial ischemia. METHODS: The left coronary arteries were ligated to create early myocardial ischemia. Cx43 was visualized by use of SP immunohistochemical method (IHC) and Colloidal gold of the electron microscopy, and a comparison was made between the experiment group and the control group. The semi-quantitation assessment was performed. RESULTS: The positive areas of Cx43 gap junctions by IHC and Colloidal gold particles of the electron microscopy were found decreased by about 40% at 30 minutes of acute myocardial ischemia. The distributions were not obviously modified. CONCLUSION: The decrease of Cx43 is characterized by the time during myocardial ischemia, and the early stage of acute myocardial ischemia in rat is of positive significance.
OBJECTIVE: To reveal gap junction connexin 43 (Cx43) in rabbit with early myocardial ischemia. METHODS: The left coronary arteries were ligated to create early myocardial ischemia. Cx43 was visualized by use of SP immunohistochemical method (IHC) and Colloidal gold of the electron microscopy, and a comparison was made between the experiment group and the control group. The semi-quantitation assessment was performed. RESULTS: The positive areas of Cx43 gap junctions by IHC and Colloidal gold particles of the electron microscopy were found decreased by about 40% at 30 minutes of acute myocardial ischemia. The distributions were not obviously modified. CONCLUSION: The decrease of Cx43 is characterized by the time during myocardial ischemia, and the early stage of acute myocardial ischemia in rat is of positive significance.