W G Zhang1, L Z Wang, Z Liu. 1. School of Stomatology, Shanghai Second Medical University. Shanghai 200011,China.
Abstract
OBJECTIVE: The expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied. METHODS: Our study applied the immunohistochemistry technique to detect the expression of bone sialoprotein, osteocalcin, osteopontin, alkaline phosphatase and type I collagen expressed in the bone matrix. RESULTS: They demonstrated that there were difference in the location and function of the collagenous and non-collagenous proteins in the development of bone matrix. CONCLUSION: The present results revealed that fluoride might enhance the proliferation activity of osteoblast precursors; simultaneously, fluoride might accelerate the phenotypic development of osteoblast and the expression of collagenous and non-collagenous proteins. As a result,low doses fluoride played a favorable role in the development of bone extracellular matrix.
OBJECTIVE: The expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied. METHODS: Our study applied the immunohistochemistry technique to detect the expression of bone sialoprotein, osteocalcin, osteopontin, alkaline phosphatase and type I collagen expressed in the bone matrix. RESULTS: They demonstrated that there were difference in the location and function of the collagenous and non-collagenous proteins in the development of bone matrix. CONCLUSION: The present results revealed that fluoride might enhance the proliferation activity of osteoblast precursors; simultaneously, fluoride might accelerate the phenotypic development of osteoblast and the expression of collagenous and non-collagenous proteins. As a result,low doses fluoride played a favorable role in the development of bone extracellular matrix.