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Literature DB >> 15070078

On the use of post-transcriptional processing elements in transgenes.

C B A Whitelaw¹, L Hiripi, E Farini, M L Opsahl, Z Bosze.

Abstract

RNA processing events modulate final productivity of a given transgene. We have evaluated a series of RNA elements for their ability to enhance alpha1-antitrypsin production in mammary cells. Our results indicate the need for a case-by-case assessment of each construct design and the occurrence of gene silencing events in vivo.

Entities: Disease Gene Species

Mesh：

Substances：
RNA

Year: 2004 PMID： 15070078 PMCID： PMC7089212 DOI： 10.1023/b:trag.0000017178.79427.2c

Source DB: PubMed Journal: Transgenic Res ISSN： 0962-8819 Impact factor: 2.788

31 in total

1. Multiple copies of beta-lactoglobulin promoter do not function as LCR.

Authors: R M James; C Neil; J Webster; S Roos; A J Clark; C B Whitelaw
Journal: Biochem Biophys Res Commun Date: 2000-05-27 Impact factor: 3.575

2. Multiple effects of genetic background on variegated transgene expression in mice.

Authors: Margaret L Opsahl; Margaret McClenaghan; Anthea Springbett; Sarah Reid; Richard Lathe; Alan Colman; C Bruce A Whitelaw
Journal: Genetics Date: 2002-03 Impact factor: 4.562

3. The 3' untranslated region of bovine preprolactin contains a transferable non-poly(A) mRNA sequence that prolongs translation.

Authors: A Vassilakos; M J Hughes; D W Andrews
Journal: FEBS Lett Date: 1995-02-13 Impact factor: 4.124

4. The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.

Authors: C Saavedra; B Felber; E Izaurralde
Journal: Curr Biol Date: 1997-09-01 Impact factor: 10.834

9. The effect of various introns and transcription terminators on the efficiency of expression vectors in various cultured cell lines and in the mammary gland of transgenic mice.

Authors: D Petitclerc; J Attal; M C Théron; M Bearzotti; P Bolifraud; G Kann; M G Stinnakre; H Pointu; C Puissant; L M Houdebine
Journal: J Biotechnol Date: 1995-06-21 Impact factor: 3.307

Review 10. Transgenic animal bioreactors.

Authors: L M Houdebine
Journal: Transgenic Res Date: 2000 Impact factor: 2.788

On the use of post-transcriptional processing elements in transgenes.

1. Multiple copies of beta-lactoglobulin promoter do not function as LCR.

2. Multiple effects of genetic background on variegated transgene expression in mice.

3. The 3' untranslated region of bovine preprolactin contains a transferable non-poly(A) mRNA sequence that prolongs translation.

4. The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.

5. Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.

6. Position-independent expression of the ovine beta-lactoglobulin gene in transgenic mice.

7. Specific ablation of thyroid follicle cells in adult transgenic mice.

8. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA.

9. The effect of various introns and transcription terminators on the efficiency of expression vectors in various cultured cell lines and in the mammary gland of transgenic mice.

Review 10. Transgenic animal bioreactors.