Literature DB >> 15065794

Electrophoretically mediated microanalysis.

Sona Nováková1, Sigrid Van Dyck, Ann Van Schepdael, Jos Hoogmartens, Zdenĕk Glatz.   

Abstract

This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.

Mesh:

Year:  2004        PMID: 15065794     DOI: 10.1016/j.chroma.2003.12.025

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN: 0021-9673            Impact factor:   4.759


  4 in total

1.  Increasing the efficiency of in-capillary electrophoretically mediated microanalysis reactions via rapid polarity switching.

Authors:  Brandi D Sanders; Rachel L Slotcavage; Diana L Scheerbaum; Christopher J Kochansky; Timothy G Strein
Journal:  Anal Chem       Date:  2005-04-15       Impact factor: 6.986

2.  Microfabricated channel array electrophoresis for characterization and screening of enzymes using RGS-G protein interactions as a model system.

Authors:  Jian Pei; John F Dishinger; David L Roman; Chetwana Rungwanitcha; Richard R Neubig; Robert T Kennedy
Journal:  Anal Chem       Date:  2008-05-09       Impact factor: 6.986

3.  Microscale Measurements of Michaelis-Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage.

Authors:  Srikanth Gattu; Cassandra L Crihfield; Lisa A Holland
Journal:  Anal Chem       Date:  2016-12-21       Impact factor: 6.986

4.  Application of affinity capillary electrophoresis for charge heterogeneity profiling of biopharmaceuticals.

Authors:  Andrei Hutanu; Steffen Kiessig; Andrea Bathke; Rolf Ketterer; Sonja Riner; Jan Olaf Stracke; Markus Wild; Bernd Moritz
Journal:  Electrophoresis       Date:  2019-10-08       Impact factor: 3.535

  4 in total

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