Literature DB >> 15063068

A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis.

Ingmar Janse1, Jasper Bok, Gabriel Zwart.   

Abstract

Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual 'double bands' that can be encountered in several applications.

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Year:  2004        PMID： 15063068     DOI： 10.1016/j.mimet.2003.12.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  37 in total

1.  Randomly amplified polymorphic DNA reveals tight links between viruses and microbes in the bathypelagic zone of the Northwestern Mediterranean Sea.

Authors:  Christian Winter; Markus G Weinbauer
Journal:  Appl Environ Microbiol       Date:  2010-08-20       Impact factor: 4.792

2.  Sequential reductive dechlorination of meta-chlorinated polychlorinated biphenyl congeners in sediment microcosms by two different Chloroflexi phylotypes.

Authors:  Sonja K Fagervold; Joy E M Watts; Harold D May; Kevin R Sowers
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

3.  Characterization of the prokaryotic diversity in cold saline perennial springs of the Canadian high Arctic.

Authors:  Nancy N Perreault; Dale T Andersen; Wayne H Pollard; Charles W Greer; Lyle G Whyte
Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

4.  Longitudinal changes in the bacterial community composition of the Danube River: a whole-river approach.

Authors:  Christian Winter; Thomas Hein; Gerhard Kavka; Robert L Mach; Andreas H Farnleitner
Journal:  Appl Environ Microbiol       Date:  2006-11-03       Impact factor: 4.792

5.  Adhesion forces and composition of planktonic and adhering oral microbiomes.

Authors:  S W Wessel; Y Chen; A Maitra; E R van den Heuvel; A M Slomp; H J Busscher; H C van der Mei
Journal:  J Dent Res       Date:  2013-11-01       Impact factor: 6.116

6.  Temporal bacterial diversity associated with metal-contaminated river sediments.

Authors:  Nicholas J Bouskill; Jill Barker-Finkel; Tamara S Galloway; Richard D Handy; Timothy E Ford
Journal:  Ecotoxicology       Date:  2009-09-22       Impact factor: 2.823

7.  Metatranscriptomic analysis of extremely halophilic viral communities.

Authors:  Fernando Santos; Mercedes Moreno-Paz; Inmaculada Meseguer; Cristina López; Ramon Rosselló-Mora; Víctor Parro; Josefa Antón
Journal:  ISME J       Date:  2011-04-14       Impact factor: 10.302

8.  Archaeal populations in hypersaline sediments underlying orange microbial mats in the Napoli mud volcano.

Authors:  Cassandre Sara Lazar; Stéphane L'haridon; Patricia Pignet; Laurent Toffin
Journal:  Appl Environ Microbiol       Date:  2011-02-18       Impact factor: 4.792

9.  Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments.

Authors:  Edward J Hilyard; Joanne M Jones-Meehan; Barry J Spargo; Russell T Hill
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

10.  Application of sequence-dependent electrophoresis fingerprinting in exploring biodiversity and population dynamics of human intestinal microbiota: what can be revealed?

Authors:  Geert Huys; Tom Vanhoutte; Peter Vandamme
Journal:  Interdiscip Perspect Infect Dis       Date:  2008-12-14
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