| Literature DB >> 15061697 |
I V Svadbina1, N V Zelinskaya, N P Kovalevskaya, L A Zheleznaya, N I Matvienko.
Abstract
Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.Entities:
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Year: 2004 PMID： 15061697 DOI： 10.1023/b:biry.0000022061.29918.8b
Source DB: PubMed Journal: Biochemistry (Mosc) ISSN： 0006-2979 Impact factor: 2.487