INTRODUCTION: We developed an experimental model in sheep femora to evaluate the process of cortical allograft incorporation. MATERIALS AND METHODS: Twenty-four sheep were divided into four groups according to the various treatments of cortical allografts as follows: fresh, frozen, autoclaved, and frozen with perforation. Periodical radiographic and histological evaluations were performed for each group. RESULTS: Perforated frozen allograft proved to be superior radiographically in the first stage to fresh, frozen, and autoclaved forms. Revascularization was demonstrated by both Spalteholz's technique and histological examination. Histological analysis also showed creeping substitution, from the host bone to the allograft, which increased the reabsorption to facilitate new bone penetration, including endochondral ossification at the host-graft interface. CONCLUSION: We believe that endochondral ossification is probably a biological event occurring routinely during the bone healing process and that the processes of incorporation of variously treated cortical allografts differ only at the early phase of implantation.
INTRODUCTION: We developed an experimental model in sheep femora to evaluate the process of cortical allograft incorporation. MATERIALS AND METHODS: Twenty-four sheep were divided into four groups according to the various treatments of cortical allografts as follows: fresh, frozen, autoclaved, and frozen with perforation. Periodical radiographic and histological evaluations were performed for each group. RESULTS: Perforated frozen allograft proved to be superior radiographically in the first stage to fresh, frozen, and autoclaved forms. Revascularization was demonstrated by both Spalteholz's technique and histological examination. Histological analysis also showed creeping substitution, from the host bone to the allograft, which increased the reabsorption to facilitate new bone penetration, including endochondral ossification at the host-graft interface. CONCLUSION: We believe that endochondral ossification is probably a biological event occurring routinely during the bone healing process and that the processes of incorporation of variously treated cortical allografts differ only at the early phase of implantation.
Authors: Noortje J Visser; Elisa S Rezaie; Patricia F Friedrich; Dimitra Kotsougiani; Alexander Y Shin; Allen T Bishop Journal: J Orthop Res Date: 2019-05-17 Impact factor: 3.494
Authors: Dimitra Kotsougiani; Caroline A Hundepool; Joost I Willems; Patricia Friedrich; Alexander Y Shin; Allen T Bishop Journal: J Vis Exp Date: 2017-08-13 Impact factor: 1.355
Authors: Thomas Kremer; Guilherme Giusti; Patricia F Friedrich; Wouter Willems; Allen T Bishop; Goetz A Giessler Journal: Microsurgery Date: 2011-11-24 Impact factor: 2.425
Authors: Mikko Larsen; Wouter F Willems; Michael Pelzer; Patricia F Friedrich; Mahrokh Dadsetan; Allen T Bishop Journal: Microsurgery Date: 2014-01-03 Impact factor: 2.425