Literature DB >> 15060055

Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB.

John S Van Arnam1, Jonathan L McMurry, May Kihara, Robert M Macnab.   

Abstract

Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15060055      PMCID： PMC412181          DOI： 10.1128/JB.186.8.2495-2498.2004

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

1.  Deletion analysis of the flagellar switch protein FliG of Salmonella.

Authors:  M Kihara; G U Miller; R M Macnab
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

2.  Role of FliJ in flagellar protein export in Salmonella.

Authors:  T Minamino; R Chu; S Yamaguchi; R M Macnab
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

Review 3.  Type III flagellar protein export and flagellar assembly.

Authors:  Robert M Macnab
Journal:  Biochim Biophys Acta       Date:  2004-11-11

4.  Components of the Salmonella flagellar export apparatus and classification of export substrates.

Authors:  T Minamino; R M Macnab
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

5.  A structural feature in the central channel of the bacterial flagellar FliF ring complex is implicated in type III protein export.

Authors:  H Suzuki; K Yonekura; K Murata; T Hirai; K Oosawa; K Namba
Journal:  J Struct Biol       Date:  1998-12-15       Impact factor: 2.867

6.  Intergenic suppression between the flagellar MS ring protein FliF of Salmonella and FlhA, a membrane component of its export apparatus.

Authors:  M Kihara; T Minamino; S Yamaguchi; R M Macnab
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

Review 7.  Type III protein secretion systems in bacterial pathogens of animals and plants.

Authors:  C J Hueck
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

8.  Domain structure of Salmonella FlhB, a flagellar export component responsible for substrate specificity switching.

Authors:  T Minamino; R M Macnab
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

9.  The genome sequence of Clostridium tetani, the causative agent of tetanus disease.

Authors:  Holger Bruggemann; Sebastian Baumer; Wolfgang Florian Fricke; Arnim Wiezer; Heiko Liesegang; Iwona Decker; Christina Herzberg; Rosa Martinez-Arias; Rainer Merkl; Anke Henne; Gerhard Gottschalk
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-27       Impact factor: 11.205

10.  Interactions among components of the Salmonella flagellar export apparatus and its substrates.

Authors:  T Minamino; R M MacNab
Journal:  Mol Microbiol       Date:  2000-03       Impact factor: 3.501
View more
  19 in total

1.  Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export.

Authors:  Tohru Minamino; Masafumi Shimada; Mayuko Okabe; Yumiko Saijo-Hamano; Katsumi Imada; May Kihara; Keiichi Namba
Journal:  J Bacteriol       Date:  2010-01-29       Impact factor: 3.490

2.  Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery.

Authors:  Jonathan L McMurry; John S Van Arnam; May Kihara; Robert M Macnab
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490

Review 3.  Process of protein transport by the type III secretion system.

Authors:  Partho Ghosh
Journal:  Microbiol Mol Biol Rev       Date:  2004-12       Impact factor: 11.056

4.  Determination of the Stoichiometry of the Complete Bacterial Type III Secretion Needle Complex Using a Combined Quantitative Proteomic Approach.

Authors:  Susann Zilkenat; Mirita Franz-Wachtel; York-Dieter Stierhof; Jorge E Galán; Boris Macek; Samuel Wagner
Journal:  Mol Cell Proteomics       Date:  2016-02-21       Impact factor: 5.911

5.  Helicobacter pylori FlhB function: the FlhB C-terminal homologue HP1575 acts as a "spare part" to permit flagellar export when the HP0770 FlhBCC domain is deleted.

Authors:  Matthew E Wand; R Elizabeth Sockett; Katy J Evans; Neil Doherty; Paul M Sharp; Kim R Hardie; Klaus Winzer
Journal:  J Bacteriol       Date:  2006-11       Impact factor: 3.490

Review 6.  On the road to structure-based development of anti-virulence therapeutics targeting the type III secretion system injectisome.

Authors:  Bronwyn J E Lyons; Natalie C J Strynadka
Journal:  Medchemcomm       Date:  2019-06-20       Impact factor: 3.597

7.  Mechanism of type-III protein secretion: Regulation of FlhA conformation by a functionally critical charged-residue cluster.

Authors:  Marc Erhardt; Paige Wheatley; Eun A Kim; Takanori Hirano; Yang Zhang; Mayukh K Sarkar; Kelly T Hughes; David F Blair
Journal:  Mol Microbiol       Date:  2017-02-28       Impact factor: 3.501

8.  YscU cleavage and the assembly of Yersinia type III secretion machine complexes.

Authors:  Kelly E Riordan; Olaf Schneewind
Journal:  Mol Microbiol       Date:  2008-04-29       Impact factor: 3.501

9.  Interaction of the extreme N-terminal region of FliH with FlhA is required for efficient bacterial flagellar protein export.

Authors:  Noritaka Hara; Yusuke V Morimoto; Akihiro Kawamoto; Keiichi Namba; Tohru Minamino
Journal:  J Bacteriol       Date:  2012-07-27       Impact factor: 3.490

10.  FliO regulation of FliP in the formation of the Salmonella enterica flagellum.

Authors:  Clive S Barker; Irina V Meshcheryakova; Alla S Kostyukova; Fadel A Samatey
Journal:  PLoS Genet       Date:  2010-09-30       Impact factor: 5.917
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.