| Literature DB >> 15058595 |
Ignacio Calleja1, María J Blanco-Príeto, Noelia Ruz, María Jesús Renedo, María Carmen Dios-Viéitez.
Abstract
A HPLC-UV method has been developed for assaying rifampicin in plasma and liver. The assay involved a liquid-liquid extraction procedure with dichloromethane-pentane (1:1). An Ultrabase-C18 column and a simple mobile phase consisting of a water (pH 2.27)-acetonitrile (40:60, v/v) mixture were used. The flow-rate was 1 ml/min and the effluent was monitored at 333 nm. Results from the HPLC analyses showed that the assay method is linear in the ranges 0.1-1 and 1-50 microg/ml for plasma, and 0.6-40 microg/g for liver. Intra- and inter-day R.S.D. were below 15% for all the sample types. Recoveries averaged 83 and 95% for plasma and liver, respectively. The method is being successfully applied to determine rifampicin in plasma and liver samples taken during pharmacokinetic studies in rats.Entities:
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Year: 2004 PMID： 15058595 DOI： 10.1016/j.chroma.2003.12.041
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759