BACKGROUND: The pathogenesis of alcoholic pancreatitis may involve the metabolism of ethanol (via oxidative and non-oxidative pathways) within the pancreas. The aims of this study were to determine the rate of non-oxidative metabolism in isolated rat pancreatic acini and to compare this to the rate of ethanol oxidation. METHODS: Pancreatic acini were isolated from male Sprague-Dawley rats and incubated with (14)C-ethanol. Radiolabelled fatty acid ethyl esters (non-oxidative metabolites) were isolated from lipid extracts by thin-layer chromatography. Radiolabelled acetate (oxidative metabolite) was isolated from the incubation medium by ion-exchange chromatography. RESULTS: Non-oxidative metabolism by isolated pancreatic acini was demonstrated. At 50 and 100 mmol/l ethanol, fatty acid ethyl ester concentrations were 49.6 +/- 13.3 and 199 +/- 93 micromol/l, respectively. These levels have previously been shown to result in tissue injury. Non-oxidative metabolism was increased 9-fold by addition of oleic acid and inhibited by the lipase inhibitor, tetrahydrolipstatin, by 91.05 +/- 1.99%. The rate of oxidative metabolism was 21-fold higher than that of non-oxidative metabolism. CONCLUSIONS: Intact pancreatic cells metabolize ethanol by the non-oxidative pathway, generating fatty acid ethyl esters at a rate sufficient to cause pancreatic damage. Oxidative metabolism of ethanol occurs at a much higher rate and may also play a role in pancreatitis. Copyright 2004 S. Karger AG, Basel and IAP
BACKGROUND: The pathogenesis of alcoholic pancreatitis may involve the metabolism of ethanol (via oxidative and non-oxidative pathways) within the pancreas. The aims of this study were to determine the rate of non-oxidative metabolism in isolated rat pancreatic acini and to compare this to the rate of ethanol oxidation. METHODS: Pancreatic acini were isolated from male Sprague-Dawley rats and incubated with (14)C-ethanol. Radiolabelled fatty acid ethyl esters (non-oxidative metabolites) were isolated from lipid extracts by thin-layer chromatography. Radiolabelled acetate (oxidative metabolite) was isolated from the incubation medium by ion-exchange chromatography. RESULTS: Non-oxidative metabolism by isolated pancreatic acini was demonstrated. At 50 and 100 mmol/l ethanol, fatty acid ethyl ester concentrations were 49.6 +/- 13.3 and 199 +/- 93 micromol/l, respectively. These levels have previously been shown to result in tissue injury. Non-oxidative metabolism was increased 9-fold by addition of oleic acid and inhibited by the lipase inhibitor, tetrahydrolipstatin, by 91.05 +/- 1.99%. The rate of oxidative metabolism was 21-fold higher than that of non-oxidative metabolism. CONCLUSIONS: Intact pancreatic cells metabolize ethanol by the non-oxidative pathway, generating fatty acid ethyl esters at a rate sufficient to cause pancreatic damage. Oxidative metabolism of ethanol occurs at a much higher rate and may also play a role in pancreatitis. Copyright 2004 S. Karger AG, Basel and IAP
Authors: Mukund P Srinivasan; Kamlesh K Bhopale; Anna A Caracheo; Samir M Amer; Shamis Khan; Lata Kaphalia; Gopalakrishnan Loganathan; Appakalai N Balamurugan; Bhupendra S Kaphalia Journal: Biochem Pharmacol Date: 2020-07-25 Impact factor: 5.858
Authors: Stephen J Pandol; Aurelia Lugea; Olga A Mareninova; Duane Smoot; Fred S Gorelick; Anna S Gukovskaya; Ilya Gukovsky Journal: Alcohol Clin Exp Res Date: 2011-02-01 Impact factor: 3.455
Authors: Phoebe A Phillips; Lu Yang; Arthur Shulkes; Alain Vonlaufen; Anne Poljak; Sonia Bustamante; Alessandra Warren; Zhihong Xu; Michael Guilhaus; Romano Pirola; Minoti V Apte; Jeremy S Wilson Journal: Proc Natl Acad Sci U S A Date: 2010-09-17 Impact factor: 11.205
Authors: Marta Herreros-Villanueva; Elizabeth Hijona; Jesus Maria Bañales; Angel Cosme; Luis Bujanda Journal: World J Gastroenterol Date: 2013-02-07 Impact factor: 5.742
Authors: Laszlo G Boros; Qinggao Deng; Stephen J Pandol; Hidekazu Tsukamoto; Vay Liang W Go; Wai-Nang Paul Lee Journal: Pancreas Date: 2009-03 Impact factor: 3.327