OBJECTIVE: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods. METHODS: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges. RESULTS: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods. CONCLUSION: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.
OBJECTIVE: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods. METHODS: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges. RESULTS: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods. CONCLUSION: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.
Authors: E López-Oviedo; A I Aller; C Martín; C Castro; M Ramirez; J M Pemán; E Cantón; C Almeida; E Martín-Mazuelos Journal: Antimicrob Agents Chemother Date: 2006-03 Impact factor: 5.191
Authors: A Espinel-Ingroff; B Arthington-Skaggs; N Iqbal; D Ellis; M A Pfaller; S Messer; M Rinaldi; A Fothergill; D L Gibbs; A Wang Journal: J Clin Microbiol Date: 2007-04-11 Impact factor: 5.948