Detection of the adulteration of traditional alcoholic beverages by the separation and determination of alprazolam, chloralhydrate and diazepam using reversed-phase high-performance liquid chromatography.

A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the separation and determination of psycotropic substances viz., alprazolam, chloral hydrate and diazepam in traditional alcoholic beverages, such as toddy, has been developed. Separation was accomplished using a reversed-phase C18 column with water-methanol-acetic acid (35:65:0.1 v/v/v) as a mobile solvent and a photo-diode array detector at 210 nm. The limits of detection of alprazolam, chloral hydrate and diazepam were determined to be 0.8, 4.5 and 0.4 microg, respectively. The validity of the method was checked by analyzing nearly 200 samples collected from different outlets by enforcement authorities, and the extent of adulteration was determined.