| Literature DB >> 15055737 |
E Pajtasz-Piasecka1, A Szyda, J Rossowska, A Krawczenko, M Indrová, P Grabarczyk, P Wysocki, A Mackiewicz, D Duś.
Abstract
Cells of transplantable MC38 colon carcinoma of C57BL/6 mice were adapted to growth in vitro as the MC38/0 cell line. Along the establishing process, MC38/0 cells preserved their tumorigenicity. After transduction with a retroviral vector carrying murine interleukin 12 (mIL-12) genes and further selection, stable MC38/IL-12 transductant cells were obtained. These cells produced IL-12 (approx. 2500 ng/ml/5x10(5) cells/48 h) as evaluated in the optimized bioassay. After subcutaneous inoculation into syngeneic mice, the IL-12-modified cells demonstrated reduced tumorigenicity as compared to parental MC38/0 cells. Mice that rejected the MC38/IL-12 tumour became protected against subsequent challenge with MC38/0 cells. The obtained data indicate that the IL-12-transduced murine colon carcinoma cells could be used both as a model tumour for the study of mechanisms of anticancer immunity and/or as an adjuvant to cancer vaccines.Entities:
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Year: 2004 PMID： 15055737
Source DB: PubMed Journal: Folia Biol (Praha) ISSN： 0015-5500 Impact factor: 0.906