AIM: The evaluation of allelic losses at the FHIT and the BRCA1 genes and at three other loci at the 17q region in a series of 34 sporadic breast cancer cases from Southern Brazil. METHODS: The samples were evaluated for loss of heterozygosity (LOH) at the FHIT and the BRCA1 genes and at three other microsatellite markers at 17q, and the findings were correlated with clinicopathological parameters. RESULTS: The BRCA1 intragenic marker, D17S855, had the highest frequency of LOH, detected in 10 of 24 informative cases, followed by the D17S579 (six of 23 informative cases), D17S806 (five of 21 informative cases), and D17S785 markers (five of 21 informative cases). LOH at the FHIT intragenic marker, D3S1300, was found in six of 25 informative cases. In four of the six cases with LOH of the FHIT gene, there was concomitant loss of the BRCA1 intragenic marker. CONCLUSIONS: The frequency of allelic losses in the FHIT and BRCA1 loci in the Southern Brazilian population is similar to that described in the general population. No correlations were found when the total LOH frequency was compared with tumour size, grade, or presence of axillary lymph node metastasis. Further studies using larger sporadic breast cancer samples and additional markers would be useful to confirm these findings, in addition to establishing more specific associations with clinicopathological parameters in this specific population.
AIM: The evaluation of allelic losses at the FHIT and the BRCA1 genes and at three other loci at the 17q region in a series of 34 sporadic breast cancer cases from Southern Brazil. METHODS: The samples were evaluated for loss of heterozygosity (LOH) at the FHIT and the BRCA1 genes and at three other microsatellite markers at 17q, and the findings were correlated with clinicopathological parameters. RESULTS: The BRCA1 intragenic marker, D17S855, had the highest frequency of LOH, detected in 10 of 24 informative cases, followed by the D17S579 (six of 23 informative cases), D17S806 (five of 21 informative cases), and D17S785 markers (five of 21 informative cases). LOH at the FHIT intragenic marker, D3S1300, was found in six of 25 informative cases. In four of the six cases with LOH of the FHIT gene, there was concomitant loss of the BRCA1 intragenic marker. CONCLUSIONS: The frequency of allelic losses in the FHIT and BRCA1 loci in the Southern Brazilian population is similar to that described in the general population. No correlations were found when the total LOH frequency was compared with tumour size, grade, or presence of axillary lymph node metastasis. Further studies using larger sporadic breast cancer samples and additional markers would be useful to confirm these findings, in addition to establishing more specific associations with clinicopathological parameters in this specific population.
Authors: P Querzoli; G Albonico; M G di Iasio; S Ferretti; R Rinaldi; A Cariello; M Pedriali; M Matteuzzi; I Maestri; I Nenci Journal: Breast Cancer Res Treat Date: 2001-03 Impact factor: 4.872
Authors: A Maitra; I I Wistuba; C Washington; A K Virmani; R Ashfaq; S Milchgrub; A F Gazdar; J D Minna Journal: Am J Pathol Date: 2001-07 Impact factor: 4.307
Authors: M van der Looij; A M Cleton-Jansen; R van Eijk; H Morreau; M van Vliet; N Kuipers-Dijkshoorn; E Oláh; C J Cornelisse; P Devilee Journal: Genes Chromosomes Cancer Date: 2000-03 Impact factor: 5.006
Authors: Y Ando; H Iwase; S Ichihara; S Toyoshima; T Nakamura; H Yamashita; T Toyama; Y Omoto; S Karamatsu; S Mitsuyama; Y Fujii; S Kobayashi Journal: Cancer Lett Date: 2000-08-11 Impact factor: 8.679
Authors: Bruce C Turner; Michelle Ottey; Drazen B Zimonjic; Magdalena Potoczek; Walter W Hauck; Edward Pequignot; Catherine L Keck-Waggoner; Cinzia Sevignani; C Marcelo Aldaz; Peter A McCue; Juan Palazzo; Kay Huebner; Nicholas C Popescu Journal: Cancer Res Date: 2002-07-15 Impact factor: 12.701
Authors: Maria Nowacka-Zawisza; Magdalena Bryś; Hanna Romanowicz-Makowska; Andrzej Kulig; Wanda M Krajewska Journal: Cell Mol Biol Lett Date: 2006-12-18 Impact factor: 5.787