BACKGROUND AND AIMS: The interaction between the gall-forming grapevine parasite, phylloxera, and the susceptible grapevine species Vitis vinifera was investigated. METHODS: Phylloxera and grapevines were cocultivated using both potted and micropropagated grapevines. Development of nodosities on primary roots was studied by microscopy and histochemistry, and nodosities were analysed for biochemical changes and changes in gene expression. KEY RESULTS: Within a nodosity, phylloxera fed at a site in the root cortex. Nodosity development was characterized by swelling of the root tissue distal to the feeding site, lack of development of a suberized endodermis, and starch and amino acid accumulation, and was eventually followed by root necrosis. No evidence of a defence response was observed in pre-necrotic nodosities, but defence-type responses were observed in tissue adjacent to necrotic regions. Changes in gene expression were not detected by northern hybridization using DNA probes encoding a range of V. vinifera transcripts. CONCLUSIONS: Nodosities on V. vinifera potentially function as nutrient reservoirs, and defence responses to phylloxera attack were not detected.
BACKGROUND AND AIMS: The interaction between the gall-forming grapevine parasite, phylloxera, and the susceptible grapevine species Vitis vinifera was investigated. METHODS: Phylloxera and grapevines were cocultivated using both potted and micropropagated grapevines. Development of nodosities on primary roots was studied by microscopy and histochemistry, and nodosities were analysed for biochemical changes and changes in gene expression. KEY RESULTS: Within a nodosity, phylloxera fed at a site in the root cortex. Nodosity development was characterized by swelling of the root tissue distal to the feeding site, lack of development of a suberized endodermis, and starch and amino acid accumulation, and was eventually followed by root necrosis. No evidence of a defence response was observed in pre-necrotic nodosities, but defence-type responses were observed in tissue adjacent to necrotic regions. Changes in gene expression were not detected by northern hybridization using DNA probes encoding a range of V. vinifera transcripts. CONCLUSIONS: Nodosities on V. vinifera potentially function as nutrient reservoirs, and defence responses to phylloxera attack were not detected.
Authors: Rachel A Burton; Helen Jenner; Luke Carrangis; Brendan Fahy; Geoffrey B Fincher; Chris Hylton; David A Laurie; Mary Parker; Darren Waite; Sonja van Wegen; Tamara Verhoeven; Kay Denyer Journal: Plant J Date: 2002-07 Impact factor: 6.417
Authors: Claude Rispe; Fabrice Legeai; Paul D Nabity; Rosa Fernández; Arinder K Arora; Patrice Baa-Puyoulet; Celeste R Banfill; Leticia Bao; Miquel Barberà; Maryem Bouallègue; Anthony Bretaudeau; Jennifer A Brisson; Federica Calevro; Pierre Capy; Olivier Catrice; Thomas Chertemps; Carole Couture; Laurent Delière; Angela E Douglas; Keith Dufault-Thompson; Paula Escuer; Honglin Feng; Astrid Forneck; Toni Gabaldón; Roderic Guigó; Frédérique Hilliou; Silvia Hinojosa-Alvarez; Yi-Min Hsiao; Sylvie Hudaverdian; Emmanuelle Jacquin-Joly; Edward B James; Spencer Johnston; Benjamin Joubard; Gaëlle Le Goff; Gaël Le Trionnaire; Pablo Librado; Shanlin Liu; Eric Lombaert; Hsiao-Ling Lu; Martine Maïbèche; Mohamed Makni; Marina Marcet-Houben; David Martínez-Torres; Camille Meslin; Nicolas Montagné; Nancy A Moran; Daciana Papura; Nicolas Parisot; Yvan Rahbé; Mélanie Ribeiro Lopes; Aida Ripoll-Cladellas; Stéphanie Robin; Céline Roques; Pascale Roux; Julio Rozas; Alejandro Sánchez-Gracia; Jose F Sánchez-Herrero; Didac Santesmasses; Iris Scatoni; Rémy-Félix Serre; Ming Tang; Wenhua Tian; Paul A Umina; Manuella van Munster; Carole Vincent-Monégat; Joshua Wemmer; Alex C C Wilson; Ying Zhang; Chaoyang Zhao; Jing Zhao; Serena Zhao; Xin Zhou; François Delmotte; Denis Tagu Journal: BMC Biol Date: 2020-07-23 Impact factor: 7.431