PURPOSE: To evaluate the effects of early systemic administration of betamethasone or cyclosporin A (CsA) on inflammatory cytokine expression and corneal damage after alkali injury. METHODS: Sixty-five Wistar rat corneas injured with 1N NaOH were divided into three groups: untreated, betamethasone-treated and CsA-treated. Both agents were administered systemically and daily during the first 7 days after injury. Interleukin (IL)-1alpha, -6 and -8 concentrations in the injured corneas were measured with ELISA at 2, 4 and 7 days after injury. Corneal damage was evaluated by scoring clinical findings. RESULTS: In untreated injured corneas, IL-1alpha, -6 and -8 were markedly elevated during the 7-day period following injury. Both betamethasone and CsA significantly suppressed IL-1alpha and IL-8 at day 4. Only betamethasone significantly suppressed IL-6 at days 4 and 7. Both agents significantly reduced corneal opacity at day 4. CONCLUSION: Early systemic administration of betamethasone or CsA after alkali injury may be of benefit by suppressing inflammatory cytokine expression in the cornea.
PURPOSE: To evaluate the effects of early systemic administration of betamethasone or cyclosporin A (CsA) on inflammatory cytokine expression and corneal damage after alkali injury. METHODS: Sixty-five Wistar rat corneas injured with 1N NaOH were divided into three groups: untreated, betamethasone-treated and CsA-treated. Both agents were administered systemically and daily during the first 7 days after injury. Interleukin (IL)-1alpha, -6 and -8 concentrations in the injured corneas were measured with ELISA at 2, 4 and 7 days after injury. Corneal damage was evaluated by scoring clinical findings. RESULTS: In untreated injured corneas, IL-1alpha, -6 and -8 were markedly elevated during the 7-day period following injury. Both betamethasone and CsA significantly suppressed IL-1alpha and IL-8 at day 4. Only betamethasone significantly suppressed IL-6 at days 4 and 7. Both agents significantly reduced corneal opacity at day 4. CONCLUSION: Early systemic administration of betamethasone or CsA after alkali injury may be of benefit by suppressing inflammatory cytokine expression in the cornea.
Authors: Uri Soiberman; Siva P Kambhampati; Tony Wu; Manoj K Mishra; Yumin Oh; Rishi Sharma; Jiangxia Wang; Abdul Elah Al Towerki; Samuel Yiu; Walter J Stark; Rangaramanujam M Kannan Journal: Biomaterials Date: 2017-02-16 Impact factor: 12.479
Authors: Naveen Radhakrishnan; Venkatesh N Prajna; Lalitha S Prajna; Anitha Venugopal; Shivanandha Narayana; Revathi Rajaraman; Guillermo Amescua; Travis C Porco; Thomas M Lietman; Jennifer Rose-Nussbaumer Journal: BMJ Open Ophthalmol Date: 2021-11-29