| Literature DB >> 15041697 |
M-P Nieh1, T A Harroun, V A Raghunathan, C J Glinka, J Katsaras.
Abstract
Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca2+) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration < or =1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be approximately 20%. The stability of the Ca2+- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, <Ro>) were found to be insensitive to the lipid concentration (Clp). However, doping the dimyristoyl/dihexanoyl system with both Ca2+ and DMPG resulted in ULVs whose <Ro> was found to be Clp dependent. The <Ro> of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.Entities:
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Year: 2004 PMID： 15041697 PMCID： PMC1304108 DOI： 10.1016/S0006-3495(04)74316-7
Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033