Ritsuko Ohata1, Atsuhiko Tada. 1. Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, 739-1, Uchio, Okayama-shi, Okayama 701-0298, Japan. ritsuko_oohata@pref.okayama.jp
Abstract
OBJECTIVE: The purpose of this study is to investigate the distribution of Mycobacterium tuberculosis genotypes, such as Beijing family and other genotypes in Okayama district, and to examine the relation between these genotype strains and prevalent strains. METHODS: The 142 M. tuberculosis strains isolated in Okayama City from January 2000 to December 2002 were subjected to IS6110-RFLP and spoligotyping for the population-based study. In addition, 13 strains having 1 to 5 IS6110 copies isolated in Okayama Prefecture excluding Okayama City were also subjected to spoligotyping. RESULTS: A total of 103 (72.5%) of 142 strains belonged to Beijing family. Furthermore, 3 of 4 groups of prevalent strains belonged to the family. Twenty one strains out of 39 rest strains could be classified into 10 shared types (STs) of 259 STs in the worldwide spoligotype database. In addition, 15 out of 26 strains from inside and outside of Okayama City having 1 to 5 IS6110 copies showed common unique spoligotype. Moreover, the age of majority patients who were infected with M. tuberculosis of the Beijing family or other genotypes were older than 60 years. CONCLUSION: It was suggested that Beijing family had been the main infection source, and the spread of strains of Beijing family and other genotypes occurred considerably in old times, and the unique genotype strains remained for a long time as peculiar strains.
OBJECTIVE: The purpose of this study is to investigate the distribution of Mycobacterium tuberculosis genotypes, such as Beijing family and other genotypes in Okayama district, and to examine the relation between these genotype strains and prevalent strains. METHODS: The 142 M. tuberculosis strains isolated in Okayama City from January 2000 to December 2002 were subjected to IS6110-RFLP and spoligotyping for the population-based study. In addition, 13 strains having 1 to 5 IS6110 copies isolated in Okayama Prefecture excluding Okayama City were also subjected to spoligotyping. RESULTS: A total of 103 (72.5%) of 142 strains belonged to Beijing family. Furthermore, 3 of 4 groups of prevalent strains belonged to the family. Twenty one strains out of 39 rest strains could be classified into 10 shared types (STs) of 259 STs in the worldwide spoligotype database. In addition, 15 out of 26 strains from inside and outside of Okayama City having 1 to 5 IS6110 copies showed common unique spoligotype. Moreover, the age of majority patients who were infected with M. tuberculosis of the Beijing family or other genotypes were older than 60 years. CONCLUSION: It was suggested that Beijing family had been the main infection source, and the spread of strains of Beijing family and other genotypes occurred considerably in old times, and the unique genotype strains remained for a long time as peculiar strains.
Authors: Go Eun Choi; Mi Hee Jang; Eun Ju Song; Seok Hoon Jeong; Jae-Seok Kim; Wee Gyo Lee; Young Uh; Kyoung Ho Roh; Hye Soo Lee; Jong Hee Shin; Nam Hee Ryoo; Young Ree Kim; Joseph Jeong; Jee Hee Kim; Sun Min Lee; Jongyoun Yi; Sang Hyun Hwang; Hyung Hoi Kim; Eun Yup Lee; Chulhun L Chang; Moon-Bum Kim; Yeong Dae Kim Journal: J Korean Med Sci Date: 2010-11-24 Impact factor: 2.153
Authors: Karine Brudey; Jeffrey R Driscoll; Leen Rigouts; Wolfgang M Prodinger; Andrea Gori; Sahal A Al-Hajoj; Caroline Allix; Liselotte Aristimuño; Jyoti Arora; Viesturs Baumanis; Lothar Binder; Patricia Cafrune; Angel Cataldi; Soonfatt Cheong; Roland Diel; Christopher Ellermeier; Jason T Evans; Maryse Fauville-Dufaux; Séverine Ferdinand; Dario Garcia de Viedma; Carlo Garzelli; Lidia Gazzola; Harrison M Gomes; M Cristina Guttierez; Peter M Hawkey; Paul D van Helden; Gurujaj V Kadival; Barry N Kreiswirth; Kristin Kremer; Milan Kubin; Savita P Kulkarni; Benjamin Liens; Troels Lillebaek; Minh Ly Ho; Carlos Martin; Christian Martin; Igor Mokrousov; Olga Narvskaïa; Yun Fong Ngeow; Ludmilla Naumann; Stefan Niemann; Ida Parwati; Zeaur Rahim; Voahangy Rasolofo-Razanamparany; Tiana Rasolonavalona; M Lucia Rossetti; Sabine Rüsch-Gerdes; Anna Sajduda; Sofia Samper; Igor G Shemyakin; Urvashi B Singh; Akos Somoskovi; Robin A Skuce; Dick van Soolingen; Elisabeth M Streicher; Philip N Suffys; Enrico Tortoli; Tatjana Tracevska; Véronique Vincent; Tommie C Victor; Robin M Warren; Sook Fan Yap; Khadiza Zaman; Françoise Portaels; Nalin Rastogi; Christophe Sola Journal: BMC Microbiol Date: 2006-03-06 Impact factor: 3.605