| Literature DB >> 15025435 |
Dmitry G Shchukin1, Amish A Patel, Gleb B Sukhorukov, Yuri M Lvov.
Abstract
A new microcontainer for DNA delivery based on biocompatible poly[beta-glucuronic acid-(1 --> 3)-N-acetyl-beta-galactosamine-6-sulfate-(1 --> 4)](chondroitin sulfate)/poly(-l-arginine) microcapsules with 40 nm thick molecularly organized shell was proposed. DNA molecules were deposited as DNA/sperimidine complex on the surface of template 4 mum core particles followed by layer-by-layer nanoassembly of protective chondroitin sulfate/poly(-l-arginine) shell. After template core dissolution, DNA molecules were captured inside microcapsules retaining a natural double-helix structure. The developed DNA encapsulation approach can be employed for targeted delivery of plasmid DNA in living cells.Entities:
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Year: 2004 PMID： 15025435 DOI： 10.1021/ja036952x
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419