Literature DB >> 1502498

A family of cross-reacting proteins secreted by Mycobacterium tuberculosis.

H G Wiker1, S Nagai, M Harboe, L Ljungqvist.   

Abstract

Cross-reactions between five proteins actively secreted by Mycobacterium tuberculosis were studied by crossed immunoelectrophoresis, SDS-PAGE with immunoblotting, and ELISA using polyclonal rabbit antisera and mouse monoclonal antibodies to the purified proteins. The monoclonal antibody HBT4 was demonstrated to react with the MPT51 protein. The 85A, 85B and 85C constituents of the M. tuberculosis and Mycobacterium bovis BCG antigen 85 complex cross-react extensively, each of the components containing component-specific as well as cross-reacting epitopes. These components also cross-reacted with MPT51 and MPT64. N-terminal sequence studies revealed striking homology at the amino acid level between 85A, 85B, 85C and MPT51. MPT64 showed less homology. In addition, striking homology was demonstrated between two different stretches within the 85B sequence and indicated between three stretches within the MPT64 molecule. Thus, a family of at least four secreted proteins with common structural features has been demonstrated in mycobacteria. MPT64 may also belong to this family.

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Year:  1992        PMID： 1502498     DOI： 10.1111/j.1365-3083.1992.tb03104.x

Source DB:  PubMed          Journal:  Scand J Immunol        ISSN： 0300-9475            Impact factor:   3.487


  20 in total

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Journal:  World J Gastroenterol       Date:  2010-05-28       Impact factor: 5.742

Review 2.  Genetic vaccination against tuberculosis.

Authors:  D B Lowrie; C L Silva; R E Tascon
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3.  A comparative approach to strategies for cloning, expression, and purification of Mycobacterium tuberculosis mycolyl transferase 85B and evaluation of immune responses in BALB/c mice.

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Journal:  Mol Biotechnol       Date:  2014-06       Impact factor: 2.695

4.  New simple and rapid test for culture confirmation of Mycobacterium tuberculosis complex: a multicenter study.

Authors:  Naoki Hasegawa; Takao Miura; Koudou Ishii; Kazuhiro Yamaguchi; Thomas H Lindner; Samuel Merritt; Janis D Matthews; Salman H Siddiqi
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5.  Evidence for absence of the MPB64 gene in some substrains of Mycobacterium bovis BCG.

Authors:  H Li; J C Ulstrup; T O Jonassen; K Melby; S Nagai; M Harboe
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

6.  The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.

Authors:  A Rambukkana; J D Burggraaf; W R Faber; M Harboe; P Teeling; S Krieg; P K Das
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

7.  Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.

Authors:  A Rambukkana; P K Das; J D Burggraaf; W R Faber; P Teeling; S Krieg; J E Thole; M Harboe
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

8.  Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens.

Authors:  B M Buddle; N A Parlane; D L Keen; F E Aldwell; J M Pollock; K Lightbody; P Andersen
Journal:  Clin Diagn Lab Immunol       Date:  1999-01

9.  Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.

Authors:  A Rambukkana; P K Das; J D Burggraaf; S Yong; W R Faber; J E Thole; M Harboe
Journal:  Infect Immun       Date:  1992-12       Impact factor: 3.441

10.  Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG.

Authors:  M Harboe; T Oettinger; H G Wiker; I Rosenkrands; P Andersen
Journal:  Infect Immun       Date:  1996-01       Impact factor: 3.441
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