OBJECTIVE: To test whether a reduction in air temperature within the clinical range [37 degrees C to 30, 100% relative humidity (RH)] altered mucus transport velocity (MTV) and ciliary beat frequency (CBF) in an in vitro ovine tracheal model. DESIGN: Controlled laboratory study. SETTING: University research laboratory. SUBJECTS: Farm-reared sheep. INTERVENTIONS: Tracheae were mounted flat in an organ bath. Krebs Henseleit bathed the serosal surface and air at 100% (RH) was passed over the mucosal surface at 4 l/min. Cilial beat frequency (CBF) was measured photo-electrically and mucus transport velocity (MTV) by timing movement. After 2 h at 37 degrees C (100% RH) the tissue was either maintained with those settings (controls), or the air temperature reduced to 34 degrees C or 30 degrees C. Tissue was taken for histology before and after each experiment. MEASUREMENTS AND RESULTS: CBF was 19.8+/-2.7 beats/s and MTV 5.7+/-2.6 mm/min in tissue exposed to air at 37 degrees C. Cilial activity continued for up to 6 h in the controls but mucus transport was more fragile. Reduction of the air temperature to either 34 degrees C or 30 degrees C led to a decrease in both CBF and MTV and, frequently, total mucociliary failure. There was a reduction in epithelial mucous cell numbers in all preparations. Tissues exposed to low temperature had additional abnormal histology. CONCLUSIONS: Delivery of inspired gas at 30 degrees C, or even 34 degrees C, with 100% RH may not be sufficient to prevent epithelial damage occurring during 6 h exposure.
OBJECTIVE: To test whether a reduction in air temperature within the clinical range [37 degrees C to 30, 100% relative humidity (RH)] altered mucus transport velocity (MTV) and ciliary beat frequency (CBF) in an in vitro ovine tracheal model. DESIGN: Controlled laboratory study. SETTING: University research laboratory. SUBJECTS: Farm-reared sheep. INTERVENTIONS: Tracheae were mounted flat in an organ bath. Krebs Henseleit bathed the serosal surface and air at 100% (RH) was passed over the mucosal surface at 4 l/min. Cilial beat frequency (CBF) was measured photo-electrically and mucus transport velocity (MTV) by timing movement. After 2 h at 37 degrees C (100% RH) the tissue was either maintained with those settings (controls), or the air temperature reduced to 34 degrees C or 30 degrees C. Tissue was taken for histology before and after each experiment. MEASUREMENTS AND RESULTS: CBF was 19.8+/-2.7 beats/s and MTV 5.7+/-2.6 mm/min in tissue exposed to air at 37 degrees C. Cilial activity continued for up to 6 h in the controls but mucus transport was more fragile. Reduction of the air temperature to either 34 degrees C or 30 degrees C led to a decrease in both CBF and MTV and, frequently, total mucociliary failure. There was a reduction in epithelial mucous cell numbers in all preparations. Tissues exposed to low temperature had additional abnormal histology. CONCLUSIONS: Delivery of inspired gas at 30 degrees C, or even 34 degrees C, with 100% RH may not be sufficient to prevent epithelial damage occurring during 6 h exposure.
Authors: F Cerpa; D Cáceres; C Romero-Dapueto; C Giugliano-Jaramillo; R Pérez; H Budini; V Hidalgo; T Gutiérrez; J Molina; J Keymer Journal: Open Respir Med J Date: 2015-06-26
Authors: Oriol Roca; Gonzalo Hernández; Salvador Díaz-Lobato; José M Carratalá; Rosa M Gutiérrez; Joan R Masclans Journal: Crit Care Date: 2016-04-28 Impact factor: 9.097
Authors: Regine Gradl; Martin Dierolf; Benedikt Günther; Lorenz Hehn; Winfried Möller; David Kutschke; Lin Yang; Martin Donnelley; Rhiannon Murrie; Alexander Erl; Tobias Stoeger; Bernhard Gleich; Klaus Achterhold; Otmar Schmid; Franz Pfeiffer; Kaye Susannah Morgan Journal: Sci Rep Date: 2018-05-01 Impact factor: 4.379