BACKGROUND: Mitochondria play a critical role in ischemia-reperfusion injury of the heart. The purpose of the present study was to analyze the intracellular region-specific functional state of mitochondria after cold ischemia-reperfusion in a rat heart transplant model. METHODS: Imaging of the mitochondrial functional state in situ in nonfixed myocardial fibers was performed by confocal microscopy of mitochondrial flavoprotein autofluorescence as redox state indicator; fluorescence of Rhod-2, a specific probe for mitochondrial calcium; and of tetramethylrhodamine ethyl ester fluorescence to monitor the mitochondrial membrane potential. RESULTS: This imaging demonstrated that, in contrast to control fibers, 10-hr heart cold storage, heterotopic cardiac transplantation, and 24-hr reperfusion result in a highly heterogeneous mitochondrial functional state (mitochondrial calcium content, redox state, and inner membrane potential), thus suggesting local permeability transitions and heterogeneous mitochondrial damage. CONCLUSIONS: Imaging of in situ mitochondria allows topologic assessment of mitochondrial defects and heterogeneity, consequently providing new insights into the mechanisms of cardiac ischemia-reperfusion injury.
BACKGROUND: Mitochondria play a critical role in ischemia-reperfusion injury of the heart. The purpose of the present study was to analyze the intracellular region-specific functional state of mitochondria after cold ischemia-reperfusion in a rat heart transplant model. METHODS: Imaging of the mitochondrial functional state in situ in nonfixed myocardial fibers was performed by confocal microscopy of mitochondrial flavoprotein autofluorescence as redox state indicator; fluorescence of Rhod-2, a specific probe for mitochondrial calcium; and of tetramethylrhodamine ethyl ester fluorescence to monitor the mitochondrial membrane potential. RESULTS: This imaging demonstrated that, in contrast to control fibers, 10-hr heart cold storage, heterotopic cardiac transplantation, and 24-hr reperfusion result in a highly heterogeneous mitochondrial functional state (mitochondrial calcium content, redox state, and inner membrane potential), thus suggesting local permeability transitions and heterogeneous mitochondrial damage. CONCLUSIONS: Imaging of in situ mitochondria allows topologic assessment of mitochondrial defects and heterogeneity, consequently providing new insights into the mechanisms of cardiac ischemia-reperfusion injury.
Authors: Danh T Tran; Scott Esckilsen; Jennifer Mulligan; Shikhar Mehrotra; Carl Atkinson; Satish N Nadig Journal: Transplantation Date: 2018-06 Impact factor: 4.939
Authors: Andrey V Kozlov; Soheyl Bahrami; Enrico Calzia; Peter Dungel; Lars Gille; Andrey V Kuznetsov; Jakob Troppmair Journal: Ann Intensive Care Date: 2011-09-26 Impact factor: 6.925
Authors: Anna J Dare; Angela Logan; Tracy A Prime; Sebastian Rogatti; Martin Goddard; Eleanor M Bolton; J Andrew Bradley; Gavin J Pettigrew; Michael P Murphy; Kourosh Saeb-Parsy Journal: J Heart Lung Transplant Date: 2015-06-11 Impact factor: 10.247