| Literature DB >> 15020779 |
Alexei Kurakin1, Susan Wu, Dale E Bredesen.
Abstract
The novel screening format, target-assisted iterative screening (TAIS), comprises a simple and rapid two-step procedure for in vitro affinity selection of specific binders from enormous molecular diversities to the target molecule of interest. This detailed protocol describes the application of TAIS to a T7 phage-displayed complementary DNA (cDNA) library with a protein domain as a target. Protocols for purification of the target as glutathione-S-transferase (GST) fusion protein and modifications of the purified target that are required for the screening complement the TAIS protocol. The described application is a method of choice for the researchers interested in the identification and characterization of novel protein-protein interactions mediated by peptide recognition domains.Mesh：
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Year: 2004 PMID： 15020779 DOI： 10.1385/1-59259-759-9:047
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745