OBJECTIVE: To evaluate the effect of interleukin-8 (IL-8) on endometrial stromal cell metalloproteinase (MMP) activity and invasiveness. DESIGN: Experimental laboratory study. SETTING: University medical center. PATIENT(S): Reproductive age women without endometriosis. INTERVENTION(S): Endometrial stromal cells were grown in culture and treated with recombinant IL-8 (0.0001-10 ng/mL) for 24 to 48 hours. Supernatants were collected for soluble assay of collagenase activity and gelatin zymography. Endometrial stromal cells were plated on 8-microm pore membranes coated with Matrigel or human simple matrix and treated with IL-8 for 48 hours. MAIN OUTCOME MEASURE(S): Collagenase activity and MMP2 and 9 activity of control vs. IL-8 treated endometrial cells, and number of endometrial cells that invade through Matrigel or human simple matrix. RESULT(S): Collagenase activity in cells treated with IL-8 (1-10 ng/mL) was higher than in control cells. On gelatin zymograms, MMP2 and MMP9 activity of endometrial stromal cells was stimulated by IL-8 treatment (1-10 ng/mL). The number of cells that invaded the Matrigel or human simple matrix was 1.7-fold higher in the group treated with 10 ng/mL of IL-8 compared with the control group. CONCLUSION(S): IL-8 increases MMP activity and invasive capability of endometrial stromal cells in culture; IL-8 may be involved in the pathogenesis of endometriosis and menstrual physiology.
OBJECTIVE: To evaluate the effect of interleukin-8 (IL-8) on endometrial stromal cell metalloproteinase (MMP) activity and invasiveness. DESIGN: Experimental laboratory study. SETTING: University medical center. PATIENT(S): Reproductive age women without endometriosis. INTERVENTION(S): Endometrial stromal cells were grown in culture and treated with recombinant IL-8 (0.0001-10 ng/mL) for 24 to 48 hours. Supernatants were collected for soluble assay of collagenase activity and gelatin zymography. Endometrial stromal cells were plated on 8-microm pore membranes coated with Matrigel or human simple matrix and treated with IL-8 for 48 hours. MAIN OUTCOME MEASURE(S): Collagenase activity and MMP2 and 9 activity of control vs. IL-8 treated endometrial cells, and number of endometrial cells that invade through Matrigel or human simple matrix. RESULT(S): Collagenase activity in cells treated with IL-8 (1-10 ng/mL) was higher than in control cells. On gelatin zymograms, MMP2 and MMP9 activity of endometrial stromal cells was stimulated by IL-8 treatment (1-10 ng/mL). The number of cells that invaded the Matrigel or human simple matrix was 1.7-fold higher in the group treated with 10 ng/mL of IL-8 compared with the control group. CONCLUSION(S): IL-8 increases MMP activity and invasive capability of endometrial stromal cells in culture; IL-8 may be involved in the pathogenesis of endometriosis and menstrual physiology.
Authors: Paul D DiMusto; Guanyi Lu; Abhijit Ghosh; Karen J Roelofs; Omar Sadiq; Brendan McEvoy; Gang Su; Adriana Laser; Castigliano M Bhamidipati; Gorav Ailawadi; Peter K Henke; Jonathan L Eliason; Gilbert R Upchurch Journal: J Surg Res Date: 2011-12-14 Impact factor: 2.192
Authors: Alexis D Greene; Jessica A Kendziorski; Jeanette M Buckholz; Liang Niu; Changchun Xie; Susan M Pinney; Katherine A Burns Journal: Reprod Toxicol Date: 2018-12-21 Impact factor: 3.143
Authors: S Nair; Y H Lee; E Rousseau; M Cam; P A Tataranni; L J Baier; C Bogardus; P A Permana Journal: Diabetologia Date: 2005-07-21 Impact factor: 10.122
Authors: Idhaliz Flores; Elizabeth Rivera; Lynnette A Ruiz; Olga I Santiago; Michael W Vernon; Caroline B Appleyard Journal: Fertil Steril Date: 2007-05 Impact factor: 7.329