BACKGROUND: Oxaliplatin (L-OHP), active in a wide range of human and animal tumours, also CDDP-resistant, possesses unique molecular characteristics of action. However, the mechanisms by which the damage induced by L-OHP triggers a death signal are not yet fully defined. MATERIALS AND METHODS: After L-OHP treatment of the HCT15 human colon cancer cell line, apoptosis was evaluated by DNA laddering detection and by flow cytometry; the effect on specific caspase-3, -8 and -9 inhibitors, mitochondrial membrane permeability transition, cytochrome C release and expression of CD95 and CD95L were also assessed. RESULTS: HCT15 cells underwent apoptosis when treated with all used drug concentrations (7-25 microM). Treatment of cells with L-OHP resulted in the activation of caspase-8, -9 and -3, in a mitochondrial membrane depolarisation, and in an increase of CD95 receptor and CD95 ligand levels. CONCLUSION: The results correlated well with the ability of L-OHP to induce apoptosis and give further insights into the mechanisms underlying the L-OHP-induced apoptosis of tumor cells.
BACKGROUND:Oxaliplatin (L-OHP), active in a wide range of human and animal tumours, also CDDP-resistant, possesses unique molecular characteristics of action. However, the mechanisms by which the damage induced by L-OHP triggers a death signal are not yet fully defined. MATERIALS AND METHODS: After L-OHP treatment of the HCT15 humancolon cancer cell line, apoptosis was evaluated by DNA laddering detection and by flow cytometry; the effect on specific caspase-3, -8 and -9 inhibitors, mitochondrial membrane permeability transition, cytochrome C release and expression of CD95 and CD95L were also assessed. RESULTS: HCT15 cells underwent apoptosis when treated with all used drug concentrations (7-25 microM). Treatment of cells with L-OHP resulted in the activation of caspase-8, -9 and -3, in a mitochondrial membrane depolarisation, and in an increase of CD95 receptor and CD95 ligand levels. CONCLUSION: The results correlated well with the ability of L-OHP to induce apoptosis and give further insights into the mechanisms underlying the L-OHP-induced apoptosis of tumor cells.
Authors: Katarzyna Gach; Jacek Szymański; Dorota Pomorska; Angelika Długosz; Jakub Modranka; Tomasz Janecki; Anna Janecka Journal: Tumour Biol Date: 2015-03-06
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Authors: Louis J Delinois; Omar De León-Vélez; Adriana Vázquez-Medina; Alondra Vélez-Cabrera; Amanda Marrero-Sánchez; Christopher Nieves-Escobar; Daniela Alfonso-Cano; Delvin Caraballo-Rodríguez; Jael Rodriguez-Ortiz; Jemily Acosta-Mercado; Josué A Benjamín-Rivera; Kiara González-González; Kysha Fernández-Adorno; Lisby Santiago-Pagán; Rafael Delgado-Vergara; Xaiomy Torres-Ávila; Andrea Maser-Figueroa; Gladimarys Grajales-Avilés; Glorimar I Miranda Méndez; Javier Santiago-Pagán; Miguel Nieves-Santiago; Vanessa Álvarez-Carrillo; Kai Griebenow; Arthur D Tinoco Journal: Inorganics (Basel) Date: 2021-11-16
Authors: D Arango; A J Wilson; Q Shi; G A Corner; M J Arañes; C Nicholas; M Lesser; J M Mariadason; L H Augenlicht Journal: Br J Cancer Date: 2004-11-29 Impact factor: 7.640