OBJECTIVE: To investigate the absorption of the water soluble components of Salvia miltiorrhiza and establish methods to determine Danshensu in rat serum after ig compound Salvia recipe. METHOD: Used liquid-liquid extraction to deal with the serum, then determined the sample by high-performance liquid chromatography. The conditions for HPLC were as the following: the mobile phase, CH3OH-CH3CN-0.5% CH3COOH (2.5:3.5:94) with a flow rate of 1.0 mL.min-1; detection wavelength, 281 nm; internal standard, 4-hydroxy-benzoic acid. RESULT: Danshensu and an unknown compound whose reserved time was little shorter than protocatechuic aldehyde were found in the rat serum after ig compound Salvia recipe, while protocatechuic aldehyde weren't found in the serum. CONCLUSION: Danshensu can be absorbed into blood after ig compound Salvia recipe.
OBJECTIVE: To investigate the absorption of the water soluble components of Salvia miltiorrhiza and establish methods to determine Danshensu in rat serum after ig compound Salvia recipe. METHOD: Used liquid-liquid extraction to deal with the serum, then determined the sample by high-performance liquid chromatography. The conditions for HPLC were as the following: the mobile phase, CH3OH-CH3CN-0.5% CH3COOH (2.5:3.5:94) with a flow rate of 1.0 mL.min-1; detection wavelength, 281 nm; internal standard, 4-hydroxy-benzoic acid. RESULT: Danshensu and an unknown compound whose reserved time was little shorter than protocatechuic aldehyde were found in the rat serum after ig compound Salvia recipe, while protocatechuic aldehyde weren't found in the serum. CONCLUSION: Danshensu can be absorbed into blood after ig compound Salvia recipe.