Literature DB >> 15013043

Measurement of the antibiotic susceptibility of Coxiella burnetii using real time PCR.

Areen Boulos1, Jean-Marc Rolain, Max Maurin, Didier Raoult.   

Abstract

The objective of this study was to determine MICs of antibiotics for two reference strains of Coxiella burnetii using real time quantitative PCR. The method was very sensitive and specific and allowed the evaluation of the doubling time of Nine Mile and Q212 strains: 37 and 15 h, respectively. Dose response curves of antibiotics were used to determine MICs. Those of doxycycline, fluoroquinolone compounds and rifampicin were in the range 1-4 mg/l. Telithromycin was the most effective macrolide compound with MICs of 1-2 mg/l. The results confirmed previous reports on the accuracy of this new method for the determination of the antibiotic susceptibility of C. burnetii and could be used for the screening of new drugs.

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Year:  2004        PMID： 15013043     DOI： 10.1016/j.ijantimicag.2003.07.007

Source DB:  PubMed          Journal:  Int J Antimicrob Agents        ISSN： 0924-8579            Impact factor:   5.283


  14 in total

Review 1.  From Q Fever to Coxiella burnetii Infection: a Paradigm Change.

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2.  Enhanced detection of Coxiella burnetii with a complementary locked primer-based real-time PCR method.

Authors:  Eun-Ju Kim; Hong Yong Kang; Kyu-Jam Hwang; Sang-Hee Park; Mi-Yeoun Park; Sungdo Park; Jin Seok Yu; Ji Sung Park; Sang Hyeon Kang; Hyuk Chu
Journal:  Mol Diagn Ther       Date:  2011-04-01       Impact factor: 4.074

3.  Evaluation of antibiotic susceptibilities of Ehrlichia canis, Ehrlichia chaffeensis, and Anaplasma phagocytophilum by real-time PCR.

Authors:  S Branger; J M Rolain; D Raoult
Journal:  Antimicrob Agents Chemother       Date:  2004-12       Impact factor: 5.191

4.  Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands.

Authors:  A de Bruin; A de Groot; L de Heer; J Bok; P R Wielinga; M Hamans; B J van Rotterdam; I Janse
Journal:  Appl Environ Microbiol       Date:  2011-07-22       Impact factor: 4.792

5.  Correlation between ratio of serum doxycycline concentration to MIC and rapid decline of antibody levels during treatment of Q fever endocarditis.

Authors:  Jean-Marc Rolain; Areen Boulos; Marie-Noëlle Mallet; Didier Raoult
Journal:  Antimicrob Agents Chemother       Date:  2005-07       Impact factor: 5.191

6.  Intrinsic fluoroquinolone resistance in Orientia tsutsugamushi.

Authors:  Wiwit Tantibhedhyangkul; Emmanouil Angelakis; Narongchai Tongyoo; Paul N Newton; Catrin E Moore; Rattanaphone Phetsouvanh; Didier Raoult; Jean-Marc Rolain
Journal:  Int J Antimicrob Agents       Date:  2010-04       Impact factor: 5.283

7.  Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of q fever in humans with spontaneous abortions.

Authors:  V M Vaidya; S V S Malik; Simranpreet Kaur; Satish Kumar; S B Barbuddhe
Journal:  J Clin Microbiol       Date:  2008-04-30       Impact factor: 5.948

8.  Q fever endocarditis in Romania: the first cases confirmed by direct sequencing.

Authors:  Ani Ioana Cotar; Daniela Badescu; Mihaela Oprea; Sorin Dinu; Otilia Banu; Dan Dobreanu; Minodora Dobreanu; Adina Ionac; Mirela Flonta; Monica Straut
Journal:  Int J Mol Sci       Date:  2011-12-20       Impact factor: 5.923

9.  Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii.

Authors:  Silke R Klee; Judith Tyczka; Heinz Ellerbrok; Tatjana Franz; Sonja Linke; Georg Baljer; Bernd Appel
Journal:  BMC Microbiol       Date:  2006-01-19       Impact factor: 3.605

10.  The genetic basis of thermal reaction norm evolution in lab and natural phage populations.

Authors:  Jennifer L Knies; Rima Izem; Katie L Supler; Joel G Kingsolver; Christina L Burch
Journal:  PLoS Biol       Date:  2006-07       Impact factor: 8.029
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