Literature DB >> 15007450

Sub-second isoelectric focusing in free flow using a microfluidic device.

Yi Xu1, Chao-Xuan Zhang, Dirk Janasek, Andreas Manz.   

Abstract

Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.

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Year:  2003        PMID: 15007450     DOI: 10.1039/b308476k

Source DB:  PubMed          Journal:  Lab Chip        ISSN: 1473-0189            Impact factor:   6.799


  14 in total

Review 1.  Molecular sieving using nanofilters: past, present and future.

Authors:  Jongyoon Han; Jianping Fu; Reto B Schoch
Journal:  Lab Chip       Date:  2007-11-26       Impact factor: 6.799

2.  Universal amplification-free molecular diagnostics by billion-fold hierarchical nanofluidic concentration.

Authors:  Wei Ouyang; Jongyoon Han
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-29       Impact factor: 11.205

3.  Broadening of analyte streams due to a transverse pressure gradient in free-flow isoelectric focusing.

Authors:  Debashis Dutta
Journal:  J Chromatogr A       Date:  2017-01-03       Impact factor: 4.759

4.  Using buffer additives to improve analyte stream stability in micro free flow electrophoresis.

Authors:  Nicholas W Frost; Michael T Bowser
Journal:  Lab Chip       Date:  2010-02-03       Impact factor: 6.799

5.  Isolation of DNA aptamers using micro free flow electrophoresis.

Authors:  Meng Jing; Michael T Bowser
Journal:  Lab Chip       Date:  2011-09-23       Impact factor: 6.799

6.  Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free-flow electrophoresis devices.

Authors:  Sarah K Anciaux; Michael T Bowser
Journal:  Electrophoresis       Date:  2019-12-27       Impact factor: 3.535

7.  Measuring aptamer equilibria using gradient micro free flow electrophoresis.

Authors:  Ryan T Turgeon; Bryan R Fonslow; Meng Jing; Michael T Bowser
Journal:  Anal Chem       Date:  2010-05-01       Impact factor: 6.986

8.  Cascaded free-flow isoelectric focusing for improved focusing speed and resolution.

Authors:  Jacob W Albrecht; Jamil El-Ali; Klavs F Jensen
Journal:  Anal Chem       Date:  2007-11-10       Impact factor: 6.986

Review 9.  Micro free-flow electrophoresis: theory and applications.

Authors:  Ryan T Turgeon; Michael T Bowser
Journal:  Anal Bioanal Chem       Date:  2009-03-17       Impact factor: 4.142

10.  Microfluidic preparative free-flow isoelectric focusing: system optimization for protein complex separation.

Authors:  Jian Wen; Erik W Wilker; Michael B Yaffe; Klavs F Jensen
Journal:  Anal Chem       Date:  2010-02-15       Impact factor: 6.986

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