Literature DB >> 15006820

L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.

Hengameh van der Kaaij1, Frank Desiere, Beat Mollet, Jacques-Edouard Germond.   

Abstract

Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.

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Year:  2004        PMID: 15006820      PMCID: PMC368417          DOI: 10.1128/AEM.70.3.1869-1873.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  33 in total

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