| Literature DB >> 15005243 |
Haruhiro Kondo1, Kazushige Kiguchi, Asami Okamura, Yoshiaki Okuma, Tomohiro Iida, Yoichi Kobayashi, Masayuki Takagi, Bunpei Ishizuka, Isamu Ishiwata.
Abstract
We successfully established a novel ovarian granulosa tumor cell line (HSOGT). The tumor tissue of the ovary was derived from a 25 year-old Japanese woman under her consent. The cell line was maintained for over 14 months, subcultured more than 73 times, and had a population doubling time of 18.9 hours. Phase contrast microscopy displayed a pavement-like arrangement without contact inhibition. The chromosome number showed a wide distribution of aneuploidy and the mode was 83; many marker chromosomes were observed. The HSOGT was also successfully xenotransplanted into nude mice. The cell line produced estradiol and has preserved some characters of granulosa cells with stable growth in vitro. We firmly believe that this cell line will be a most useful tool for endocrinological investigation of human granulosa cells.Entities:
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Year: 2003 PMID： 15005243 DOI： 10.1111/j.1749-0774.2003.tb00144.x
Source DB: PubMed Journal: Hum Cell ISSN： 0914-7470 Impact factor: 4.174