Literature DB >> 1500493

Direct detection of hepatitis B virus from dried blood spots by polymerase chain reaction amplification.

B P Gupta1, N Jayasuryan, S Jameel.   

Abstract

The presence of hepatitis B virus DNA in the sera of individuals is the most definitive marker of an active viral infection. We have used polymerase chain reaction detection of hepatitis B virus DNA directly on whole blood dried as a spot on filter paper. The method is rapid, specific, and sensitive and has the ability to detect as little as 10 virus particles by ethidium bromide staining of the polymerase chain reaction-amplified products. The method is cost-effective, and the stability of the spots makes the collection and transportation of potentially infectious blood safe.

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Year:  1992        PMID： 1500493      PMCID： PMC265415          DOI： 10.1128/jcm.30.8.1913-1916.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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Review 2.  The molecular biology of the hepatitis B viruses.

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Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

3.  Sickle-cell anemia and other hemoglobinopathies. Procedures and strategy for screening employing spots of blood on filter paper as specimens.

Authors:  M D Garrick; P Dembure; R Guthrie
Journal:  N Engl J Med       Date:  1973-06-14       Impact factor: 91.245

4.  Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction.

Authors:  T A Landers; H B Greenberg; W S Robinson
Journal:  J Virol       Date:  1977-08       Impact factor: 5.103

5.  Screening for PKU in sick or premature neonates.

Authors:  E R McCabe; L McCabe
Journal:  J Pediatr       Date:  1983-09       Impact factor: 4.406

6.  Detection of hepatitis B virus DNA in liver and serum: a direct appraisal of the chronic carrier state.

Authors:  C Brechot; M Hadchouel; J Scotto; F Degos; P Charnay; C Trepo; P Tiollais
Journal:  Lancet       Date:  1981-10-10       Impact factor: 79.321

7.  Detection and direct sequencing of hepatitis B virus genome by DNA amplification method.

Authors:  O Yokosuka; M Omata; K Hosoda; M Tada; T Ehata; M Ohto
Journal:  Gastroenterology       Date:  1991-01       Impact factor: 22.682

8.  Use of dried blood spot specimens in the detection of human immunodeficiency virus type 1 by the polymerase chain reaction.

Authors:  S Cassol; T Salas; M Arella; P Neumann; M T Schechter; M O'Shaughnessy
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

9.  An automatic modified polymerase chain reaction procedure for hepatitis B virus DNA detection.

Authors:  D Larzul; D Chevrier; V Thiers; J L Guesdon
Journal:  J Virol Methods       Date:  1990-01       Impact factor: 2.014

10.  Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay.

Authors:  S Kaneko; R H Miller; S M Feinstone; M Unoura; K Kobayashi; N Hattori; R H Purcell
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205
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  14 in total

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Journal:  J Clin Microbiol       Date:  2012-09-19       Impact factor: 5.948

4.  A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.

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5.  Clinical presentation and genotype of hepatitis delta in Karachi.

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6.  Detection of duck hepatitis B virus DNA on filter paper by PCR and SYBR green dye-based quantitative PCR.

Authors:  Chi-Young J Wang; Joseph J Giambrone; Bruce F Smith
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

7.  Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers.

Authors:  Laura Mercatali; Patrizia Serra; Giacomo Miserocchi; Chiara Spadazzi; Chiara Liverani; Alessandro De Vita; Giorgia Marisi; Alberto Bongiovanni; Federica Recine; Akwilina Pangan; Nestory Masalu; Toni Ibrahim; Dino Amadori
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8.  Detection of infections with hepatitis B virus, hepatitis C virus, and human immunodeficiency virus by analyses of dried blood spots--performance characteristics of the ARCHITECT system and two commercial assays for nucleic acid amplification.

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9.  Dried blood spot sampling for hepatitis B virus serology and molecular testing.

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10.  Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA.

Authors:  Aline S Ferraz; Elza F T Belo; Ligia M C C Coutinho; Ana P Oliveira; Andréia M S Carmo; Daniele L Franco; Tatiane Ferreira; André Y Yto; Marta S F Machado; Monica C G Scola; Elizabeth De Gaspari
Journal:  BMC Infect Dis       Date:  2008-03-06       Impact factor: 3.090
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