Literature DB >> 15004836

Diffusion constant in gel electrophoresis at high fields.

Małgorzata J Krawczyk1, Józef Dulak, Paweł Paściak, Krzysztof Kułakowski.   

Abstract

We present new measurements of the diffusion constant D in standard (slab-gel) electrophoresis of DNA at fields up to 10 V/cm. Molecules investigated are bacteriophages: T4 of length 173 kbp and lambda of length 48.5 kbp cut by restriction enzyme HindIII. We show, that D increases with the molecule length for electric field E above 5 V/cm. The results are interpreted within the geometration model.

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Year:  2004        PMID: 15004836     DOI: 10.1002/elps.200305678

Source DB:  PubMed          Journal:  Electrophoresis        ISSN: 0173-0835            Impact factor:   3.535


  2 in total

1.  Continuous-time random walk models of DNA electrophoresis in a post array: part II. Mobility and sources of band broadening.

Authors:  Daniel W Olson; Sarit Dutta; Nabil Laachi; Mingwei Tian; Kevin D Dorfman
Journal:  Electrophoresis       Date:  2011-02-03       Impact factor: 3.535

2.  Diffusion of DNA molecules in gel at high electric fields.

Authors:  P Pasciak; M J Krawczyk; E Gudowska-Nowak; K Kulakowski
Journal:  J Biol Phys       Date:  2005-12       Impact factor: 1.365

  2 in total

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