Literature DB >> 15004100

Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood.

Shannon E Nesbitt1, Linda Cook, Keith R Jerome.   

Abstract

A previous study suggested that delayed sample preparation can cause a false-positive plasma cytomegalovirus (CMV) PCR. We evaluated 78 samples submitted for CMV testing, and we report that a 24-h delay in plasma separation did not affect viral quantitation by real-time PCR. Laboratories should evaluate specimen handling requirements for their own PCR technology.

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Year:  2004        PMID： 15004100      PMCID： PMC356862          DOI： 10.1128/JCM.42.3.1296-1297.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR.

Authors:  Michael Boeckh; MeeiLi Huang; James Ferrenberg; Terry Stevens-Ayers; Laurence Stensland; W Garrett Nichols; Lawrence Corey
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

2.  Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented.

Authors:  René Boom; Cees J A Sol; Tim Schuurman; Alex Van Breda; Jan F L Weel; Marcel Beld; Ineke J M Ten Berge; Pauline M E Wertheim-Van Dillen; Menno D De Jong
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

Review 3.  Recent advances in the therapy and prevention of CMV infections.

Authors:  W G Nichols; M Boeckh
Journal:  J Clin Virol       Date:  2000-02       Impact factor: 3.168

4.  False-positive results of plasma PCR for cytomegalovirus DNA due to delayed sample preparation.

Authors:  P Schäfer; W Tenschert; M Schröter; K Gutensohn; R Laufs
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948
  4 in total
  4 in total

Review 1.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

2.  Cytomegalovirus DNA stability in EDTA anti-coagulated whole blood and plasma samples.

Authors:  Deborah Abdul-Ali; Colleen S Kraft; Jessica Ingersoll; Mona Frempong; Angela M Caliendo
Journal:  J Clin Virol       Date:  2011-09-06       Impact factor: 3.168

3.  Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load.

Authors:  Ajit P Limaye; Tracy K Santo Hayes; Meei-Li Huang; Amalia Magaret; Michael Boeckh; Keith R Jerome
Journal:  J Clin Microbiol       Date:  2013-05-15       Impact factor: 5.948

4.  Prediction of cytomegalovirus (CMV) plasma load from evaluation of CMV whole-blood load in samples from renal transplant recipients.

Authors:  Isabelle Garrigue; Adélaïde Doussau; Julien Asselineau; Hélène Bricout; Lionel Couzi; Catherine Rio; Pierre Merville; Hervé Fleury; Marie-Edith Lafon; Rodolphe Thiébaut
Journal:  J Clin Microbiol       Date:  2007-12-05       Impact factor: 5.948
  4 in total

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