Literature DB >> 15004095

Evaluation of penicillin binding protein 2a latex agglutination assay for identification of methicillin-resistant Staphylococcus aureus directly from blood cultures.

Kimberle C Chapin1, Michael C Musgnug.   

Abstract

The penicillin binding protein 2a (PBP2a) latex agglutination test using a blood culture pellet was compared to the oxacillin screen agar method using isolated colonies. For blood cultures positive for Staphylococcus aureus (n = 70), the direct PBP2a test was 18% sensitive and 100% specific. The PBP2a test shows poor sensitivity when used directly with positive blood cultures.

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Year:  2004        PMID： 15004095      PMCID： PMC356841          DOI： 10.1128/JCM.42.3.1283-1284.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Direct susceptibility testing of positive blood cultures by using Sensititre broth microdilution plates.

Authors:  Kimberle C Chapin; Michael C Musgnug
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

2.  A rapid latex agglutination assay for the detection of penicillin-binding protein 2'.

Authors:  Y Nakatomi; J Sugiyama
Journal:  Microbiol Immunol       Date:  1998       Impact factor: 1.955

3.  Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains.

Authors:  G Sakoulas; H S Gold; L Venkataraman; P C DeGirolami; G M Eliopoulos; Q Qian
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

4.  Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus.

Authors:  M Cavassini; A Wenger; K Jaton; D S Blanc; J Bille
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

5.  Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing.

Authors:  J Barenfanger; C Drake; G Kacich
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

6.  Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test.

Authors:  W B van Leeuwen; C van Pelt; A Luijendijk; H A Verbrugh; W H Goessens
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

7.  Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus.

Authors:  A van Griethuysen; M Pouw; N van Leeuwen; M Heck; P Willemse; A Buiting; J Kluytmans
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

8.  Clinical impact of rapid in vitro susceptibility testing and bacterial identification.

Authors:  G V Doern; R Vautour; M Gaudet; B Levy
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948
  8 in total
  9 in total

1.  Identification of Staphylococcus species directly from positive blood culture broth by use of molecular and conventional methods.

Authors:  Maitry S Mehta; Suzanne M Paule; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  J Clin Microbiol       Date:  2009-02-11       Impact factor: 5.948

2.  Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test.

Authors:  Qinfang Qian; Lata Venkataraman; James E Kirby; Howard S Gold; Toshiaki Yamazumi
Journal:  J Clin Microbiol       Date:  2010-02-03       Impact factor: 5.948

3.  Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus.

Authors:  Melissa B Miller; Heather Meyer; Elaine Rogers; Peter H Gilligan
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

4.  Anticoagulant carryover may influence clot formation in direct tube coagulase tests from blood cultures.

Authors:  Kerry Varettas; Chinmoy Mukerjee; Peter C Taylor
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

5.  Evaluation of Bio-Rad MRSASelect agar for detection of methicillin-resistant Staphylococcus aureus directly from blood cultures.

Authors:  Stefan Riedel; Lisa Dam; Paul D Stamper; Syed A R Shah; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2010-04-14       Impact factor: 5.948

6.  Rapid differentiation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by flow cytometry after brief antibiotic exposure.

Authors:  Nabin K Shrestha; Nikole M Scalera; Deborah A Wilson; Gary W Procop
Journal:  J Clin Microbiol       Date:  2011-04-06       Impact factor: 5.948

7.  Rapid identification of methicillin-resistant staphylococci bacteremia among intensive care unit patients.

Authors:  Manal Diab; Mervat El-Damarawy; Mouhamed Shemis
Journal:  Medscape J Med       Date:  2008-05-28

8.  Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears.

Authors:  Raymond Ruimy; Marie Dos-Santos; Laurent Raskine; Frédéric Bert; René Masson; Sandrine Elbaz; Christine Bonnal; Jean-Christophe Lucet; Agnès Lefort; Bruno Fantin; Michel Wolff; Michele Hornstein; Antoine Andremont
Journal:  J Clin Microbiol       Date:  2008-04-16       Impact factor: 5.948

Review 9.  Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.

Authors:  Hagen Frickmann; Wycliffe Omurwa Masanta; Andreas E Zautner
Journal:  Biomed Res Int       Date:  2014-09-17       Impact factor: 3.411
  9 in total

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