Literature DB >> 150022

In vitro reactivity of human lymphocytes after cryopreservation using a programmed cooling device.

M J Du Bois, P T Schellekens, J J De Wit, V P Eijsvoogel.   

Abstract

In this article a technique is described for the cryopreservation of human lymphocytes employing a programmed cooling device. A preset temperature curve was used which compensated for the heat exchange during crystallization. The influence of this method of lymphocyte preservation on a variety of the in vitro functions of these cells was evaluated by means such as the lymphocyte transformation test, CML and investigations for lymphocyte membrane markers. All the lymphocyte functions that were tested were found to be retained in full.

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Year:  1976        PMID: 150022     DOI: 10.1111/j.1365-3083.1976.tb03852.x

Source DB:  PubMed          Journal:  Scand J Immunol        ISSN: 0300-9475            Impact factor:   3.487


  4 in total

1.  The influence of prednisolone on the recirculation of peripheral blood lymphocytes in vivo.

Authors:  E Bloemena; S Weinreich; P T Schellekens
Journal:  Clin Exp Immunol       Date:  1990-06       Impact factor: 4.330

2.  Natural killer (NK)-cell activity in sorted subsets of peripheral blood mononuclear cells from patients with severe combined immunodeficiency.

Authors:  R J ten Berge; P T Schellekens; A Budding-Koppenol; L J Dooren; J M Vossen
Journal:  J Clin Immunol       Date:  1987-05       Impact factor: 8.317

3.  Lymphocyte transformation test in leprosy: decreased lymphocyte reactivity to Mycobacterium leprae in lepromatous leprosy, with no evidence for a generalized impairment.

Authors:  W R Faber; D L Leiker; I M Nengerman; W P Zeijlemaker; P T Schellekens
Journal:  Infect Immun       Date:  1978-12       Impact factor: 3.441

4.  Changes in human T lymphocytes after thymectomy and during senescence.

Authors:  R J Van de Griend; M Carreno; R Van Doorn; C J Leupers; A Van den Ende; P Wijermans; H J Oosterhuis; A Astaldi
Journal:  J Clin Immunol       Date:  1982-10       Impact factor: 8.317

  4 in total

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