AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION: TGF beta and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.
AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION:TGF beta and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.
Authors: Malayannan Subramaniam; Genevieve Gorny; Steven A Johnsen; David G Monroe; Glenda L Evans; Daniel G Fraser; David J Rickard; Kay Rasmussen; Jan M A van Deursen; Russell T Turner; Merry Jo Oursler; Thomas C Spelsberg Journal: Mol Cell Biol Date: 2005-02 Impact factor: 4.272
Authors: Malayannan Subramaniam; John R Hawse; Nalini M Rajamannan; James N Ingle; Thomas C Spelsberg Journal: Biofactors Date: 2010 Jan-Feb Impact factor: 6.113