BACKGROUND: The extend potential applications of a nonradioactive method for measuring circulating RBC volume, we tested the hypothesis that RBC volume could be determined independently using two populations of RBCs labeled with low-density biotin (LDB1) and high-density biotin (HDB). STUDY DESIGN AND METHODS: In 10 healthy adults, autologous RBCs were labeled with HDB, LDB, or 51Cr. The labeled RBCs were mixed and transfused. RBC volume was measured in postinfusion peripheral venous blood by quantitating dilution of each population of labeled RBCs. RESULTS: RBC volume measured using either LDB or HDB cells agreed well with RBC volume measured using 51Cr. For the regression of RBC volume by LDB versus RBC volume by 51Cr, correlation = 0.994 and slope = 0.933. For HDB versus 51Cr, correlation = 0.982 and slope = 0.953. RBC volume measured a second time in four subjects with HDB agreed well; mean CV for the differences between HDB and 51Cr were less than 5 percent. CONCLUSIONS: Using RBCs labeled with two different densities of biotin, RBC volume can be accurately measured simultaneously and repeatedly in the same subject without radiation exposure.
BACKGROUND: The extend potential applications of a nonradioactive method for measuring circulating RBC volume, we tested the hypothesis that RBC volume could be determined independently using two populations of RBCs labeled with low-density biotin (LDB1) and high-density biotin (HDB). STUDY DESIGN AND METHODS: In 10 healthy adults, autologous RBCs were labeled with HDB, LDB, or 51Cr. The labeled RBCs were mixed and transfused. RBC volume was measured in postinfusion peripheral venous blood by quantitating dilution of each population of labeled RBCs. RESULTS: RBC volume measured using either LDB or HDB cells agreed well with RBC volume measured using 51Cr. For the regression of RBC volume by LDB versus RBC volume by 51Cr, correlation = 0.994 and slope = 0.933. For HDB versus 51Cr, correlation = 0.982 and slope = 0.953. RBC volume measured a second time in four subjects with HDB agreed well; mean CV for the differences between HDB and 51Cr were less than 5 percent. CONCLUSIONS: Using RBCs labeled with two different densities of biotin, RBC volume can be accurately measured simultaneously and repeatedly in the same subject without radiation exposure.
Authors: Donald M Mock; Demet Nalbant; Svetlana V Kyosseva; Robert L Schmidt; Guohua An; Nell I Matthews; Alexander P J Vlaar; Robin van Bruggen; Dirk de Korte; Ronald G Strauss; José A Cancelas; Robert S Franco; Peter Veng-Pedersen; John A Widness Journal: Transfusion Date: 2018-05-16 Impact factor: 3.157
Authors: Donald M Mock; Nell I Matthews; Shan Zhu; Leon F Burmeister; M Bridget Zimmerman; Ronald G Strauss; Robert L Schmidt; Demet Nalbant; Kevin J Freise; Peter Veng-Pedersen; John A Widness Journal: Transfusion Date: 2010-12 Impact factor: 3.157
Authors: Paramjit K Khera; Eric P Smith; Christopher J Lindsell; Mary Colleen Rogge; Shannon Haggerty; David A Wagner; Mary B Palascak; Shilpa Mehta; Jacqueline M Hibbert; Clinton H Joiner; Robert S Franco; Robert M Cohen Journal: Am J Hematol Date: 2015-01 Impact factor: 10.047
Authors: Donald M Mock; Nell I Matthews; Shan Zhu; Ronald G Strauss; Robert L Schmidt; M Bridget Zimmerman; Demet Nalbant; Kevin J Freise; Mohammad Saleh; Peter Veng-Pedersen; John A Widness Journal: Transfusion Date: 2012-01-09 Impact factor: 3.157
Authors: Donald M Mock; John A Widness; Peter Veng-Pedersen; Ronald G Strauss; Jose A Cancelas; Robert M Cohen; Christopher J Lindsell; Robert S Franco Journal: Transfus Med Rev Date: 2014-04-05
Authors: Donald M Mock; Nell I Mock; Gary L Lankford; Leon F Burmeister; Ronald G Strauss; John A Widness Journal: Pediatr Res Date: 2008-11 Impact factor: 3.756
Authors: Donald M Mock; Nell I Matthews; Ronald G Strauss; Leon F Burmeister; Robert Schmidt; John A Widness Journal: Transfusion Date: 2009-02-10 Impact factor: 3.157