AIM: To study the diagnostic significance of K-ras gene mutations in fecal samples from elderly patients with large intestinal cancer. METHODS: DNA was extracted in the fecal and tissue samples from 23 large intestinal cancer patients, 20 colonic adenomatoid polypus patients and 20 healthy subjects. The K-ras gene mutations at the first and second bases of codon 12 were detected by the allele specific mismatch method. RESULTS: The K-ras gene mutation was 56.52%(13/23) in the large intestinal cancer patients, which was notably higher than that in the normal subjects whose K-ras gene mutation was 5%(1/20) (chi (2)=12.93, P<0.001). There was no significant difference in comparison with that of colonic adenomatoid polypus patients whose K-ras gene mutation was 30%(6/12) (chi (2)=3.05, P>0.05). The K-ras gene mutation at the second base of codon 12 was 92.13%(12/13) in the large intestinal cancer patients. There was no significant difference between the detection rate of K-ras gene mutation in the fecal and tissue samples (chi (2)=9.35, P<0.01). CONCLUSION: Our results indicate that detection of the K-ras gene mutations in fecal samples provides a non-invasive diagnostic method for the elderly large intestinal cancer patients. Its significance in the early diagnosis of large intestinal cancer awaits further studies.
AIM: To study the diagnostic significance of K-ras gene mutations in fecal samples from elderly patients with large intestinal cancer. METHODS: DNA was extracted in the fecal and tissue samples from 23 large intestinal cancerpatients, 20 colonic adenomatoid polypuspatients and 20 healthy subjects. The K-ras gene mutations at the first and second bases of codon 12 were detected by the allele specific mismatch method. RESULTS: The K-ras gene mutation was 56.52%(13/23) in the large intestinal cancerpatients, which was notably higher than that in the normal subjects whose K-ras gene mutation was 5%(1/20) (chi (2)=12.93, P<0.001). There was no significant difference in comparison with that of colonic adenomatoid polypuspatients whose K-ras gene mutation was 30%(6/12) (chi (2)=3.05, P>0.05). The K-ras gene mutation at the second base of codon 12 was 92.13%(12/13) in the large intestinal cancerpatients. There was no significant difference between the detection rate of K-ras gene mutation in the fecal and tissue samples (chi (2)=9.35, P<0.01). CONCLUSION: Our results indicate that detection of the K-ras gene mutations in fecal samples provides a non-invasive diagnostic method for the elderly large intestinal cancerpatients. Its significance in the early diagnosis of large intestinal cancer awaits further studies.
Authors: J S Mandel; T R Church; J H Bond; F Ederer; M S Geisser; S J Mongin; D C Snover; L M Schuman Journal: N Engl J Med Date: 2000-11-30 Impact factor: 91.245
Authors: Manon van Engeland; Guido M J M Roemen; Mirian Brink; Marco M M Pachen; Matty P Weijenberg; Adriaan P de Bruïne; Jan-Willem Arends; Piet A van den Brandt; Anton F P M de Goeij; James G Herman Journal: Oncogene Date: 2002-05-23 Impact factor: 9.867
Authors: C Ratto; G Flamini; L Sofo; P Nucera; M Ippoliti; G Curigliano; G Ferretti; A Sgambato; M Merico; G B Doglietto; A Cittadini; F Crucitti Journal: Dis Colon Rectum Date: 1996-11 Impact factor: 4.585
Authors: Michael Pignone; Melissa Rich; Steven M Teutsch; Alfred O Berg; Kathleen N Lohr Journal: Ann Intern Med Date: 2002-07-16 Impact factor: 25.391
Authors: Mario Hermsen; Cindy Postma; Jan Baak; Marjan Weiss; Anna Rapallo; Andrea Sciutto; Guido Roemen; Jan-Willem Arends; Richard Williams; Walter Giaretti; Anton De Goeij; Gerrit Meijer Journal: Gastroenterology Date: 2002-10 Impact factor: 22.682