OBJECTIVE: To determine the temporal and spatial expression of interleukins (IL)-13 and IL-15 in ovarian carcinoma compared to normal ovarian tissue. METHODS: Quantitative RT-PCR, ELISA and immunohistochemistry. RESULTS: Q-RT-PCR, ELISA and immunohistochemical analysis indicates that IL-13 and IL-15 mRNA and protein are expressed in normal ovary at various phases of the menstrual cycle with immunoreactive proteins detected in granulosa/theca and luteal cells and to a lesser extent in stromal cells and surface epithelial cells. Compared to normal ovary, ovarian carcinoma expresses elevated levels of IL-13 and IL-15 mRNA, with higher IL-13 expression in primary vs. metastatic tumors. IL-13 and IL-15 protein expression was also higher in the tumor tissues compared to ascites. In normal ovary, ovarian tumors and ascites, the ratio of IL-13/IL-15 favored IL-13. Immunoreactive IL-13 and IL-15 proteins were localized primarily in the tumor cells and infiltrated inflammatory cells with increased intensity with disease stage. CONCLUSION: Normal ovary and ovarian tumors express IL-13 and IL-15 and pattern of their expression in carcinomas suggests that these cytokines may function in various ovarian cellular activities including inflammatory/immune responses that are integrated cellular events taking place in normal ovary and ovarian tumors.
OBJECTIVE: To determine the temporal and spatial expression of interleukins (IL)-13 and IL-15 in ovarian carcinoma compared to normal ovarian tissue. METHODS: Quantitative RT-PCR, ELISA and immunohistochemistry. RESULTS: Q-RT-PCR, ELISA and immunohistochemical analysis indicates that IL-13 and IL-15 mRNA and protein are expressed in normal ovary at various phases of the menstrual cycle with immunoreactive proteins detected in granulosa/theca and luteal cells and to a lesser extent in stromal cells and surface epithelial cells. Compared to normal ovary, ovarian carcinoma expresses elevated levels of IL-13 and IL-15 mRNA, with higher IL-13 expression in primary vs. metastatic tumors. IL-13 and IL-15 protein expression was also higher in the tumor tissues compared to ascites. In normal ovary, ovarian tumors and ascites, the ratio of IL-13/IL-15 favored IL-13. Immunoreactive IL-13 and IL-15 proteins were localized primarily in the tumor cells and infiltrated inflammatory cells with increased intensity with disease stage. CONCLUSION:Normal ovary and ovarian tumors express IL-13 and IL-15 and pattern of their expression in carcinomas suggests that these cytokines may function in various ovarian cellular activities including inflammatory/immune responses that are integrated cellular events taking place in normal ovary and ovarian tumors.
Authors: C B Mathias; C M Schramm; L A Guernsey; C A Wu; S H Polukort; J Rovatti; J Ser-Dolansky; E Secor; S S Schneider; R S Thrall; H L Aguila Journal: Clin Exp Allergy Date: 2017-02-07 Impact factor: 5.018
Authors: Tess V Clendenen; Eva Lundin; Anne Zeleniuch-Jacquotte; Karen L Koenig; Franco Berrino; Annekatrin Lukanova; Anna E Lokshin; Annika Idahl; Nina Ohlson; Goran Hallmans; Vittorio Krogh; Sabina Sieri; Paola Muti; Adele Marrangoni; Brian M Nolen; Mengling Liu; Roy E Shore; Alan A Arslan Journal: Cancer Epidemiol Biomarkers Prev Date: 2011-04-05 Impact factor: 4.254