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Literature DB >> 14984322

Fluorescence competition assay for the assessment of ATP binding to an isolated domain of Na+, K(+)-ATPase.

Abstract

An equation allowing estimation of the dissociation constant for binding of a non-fluorescent ligand to the enzyme is presented that is based on the competitive replacement of the ligand by its fluorescent analog. We derived an explicit formula for the probe fluorescence intensity, which is suitable for nonlinear least-squares analysis. We used this formula to evaluate the binding of ATP to the large cytoplasmic loop of Na+,K(+)-ATPase. The estimated value of KD (6.2+/- 0.7 mM) is comparable with the results from other laboratories for similar constructs obtained by a different method.

Entities: Chemical

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Year: 2004 PMID： 14984322

Source DB: PubMed Journal: Physiol Res ISSN： 0862-8408 Impact factor: 1.881

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Cited

6 in total

1. Anopheles gambiae odorant binding protein crystal complex with the synthetic repellent DEET: implications for structure-based design of novel mosquito repellents.

Authors: K E Tsitsanou; T Thireou; C E Drakou; K Koussis; M V Keramioti; D D Leonidas; E Eliopoulos; K Iatrou; S E Zographos
Journal: Cell Mol Life Sci Date: 2011-06-14 Impact factor: 9.261

2. Myo1c binds tightly and specifically to phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate.

Authors: David E Hokanson; E Michael Ostap
Journal: Proc Natl Acad Sci U S A Date: 2006-02-21 Impact factor: 11.205

3. Changes in electrostatic surface potential of Na+/K+-ATPase cytoplasmic headpiece induced by cytoplasmic ligand(s) binding.

Authors: Martin Kubala; Lenka Grycova; Zdenek Lansky; Petr Sklenovsky; Marika Janovska; Michal Otyepka; Jan Teisinger
Journal: Biophys J Date: 2009-09-16 Impact factor: 4.033

Fluorescence competition assay for the assessment of ATP binding to an isolated domain of Na+, K(+)-ATPase.

1. Anopheles gambiae odorant binding protein crystal complex with the synthetic repellent DEET: implications for structure-based design of novel mosquito repellents.

2. Myo1c binds tightly and specifically to phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate.

3. Changes in electrostatic surface potential of Na+/K+-ATPase cytoplasmic headpiece induced by cytoplasmic ligand(s) binding.

4. Isotope-coded ATP probe for quantitative affinity profiling of ATP-binding proteins.

5. Identification of cisplatin-binding sites on the large cytoplasmic loop of the Na⁺/K⁺-ATPase.

6. Flavonolignans As a Novel Class of Sodium Pump Inhibitors.

Fluorescence competition assay for the assessment of ATP binding to an isolated domain of Na+, K(+)-ATPase.

1. Anopheles gambiae odorant binding protein crystal complex with the synthetic repellent DEET: implications for structure-based design of novel mosquito repellents.

2. Myo1c binds tightly and specifically to phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate.

3. Changes in electrostatic surface potential of Na+/K+-ATPase cytoplasmic headpiece induced by cytoplasmic ligand(s) binding.

4. Isotope-coded ATP probe for quantitative affinity profiling of ATP-binding proteins.

5. Identification of cisplatin-binding sites on the large cytoplasmic loop of the Na+/K+-ATPase.

6. Flavonolignans As a Novel Class of Sodium Pump Inhibitors.

5. Identification of cisplatin-binding sites on the large cytoplasmic loop of the Na⁺/K⁺-ATPase.