Yuan-fei Fu1, De-min Chen, Jian-zhong Zhang. 1. Department of Prosthodontics, Ninth People's Hospital, Shanghai Second Medical University 200011, Shanghai, China.
Abstract
OBJECTIVE: To evaluate the cytotoxicity of strontium substituted hydroxyapatite. METHODS: Cell Relative Growth Rate(RGR) method, MTT assay and Flow Cytometry(FCM) method were used, and the strontium substituted hydroxyapatite contained different strontium concentration(0%,1%,5%,10%,100%). RESULTS: It was found that there's no apparent cytotoxicity of all the strontium substituted hydroxyapatite,but the cytotoxicity increased as the strontium concentration raised. As the FCM method appeared, there was no apparent difference between the pure hydroxyapatite and 1%,5% strontium substituted hydroxyapatite. CONCLUSION: Strontium substituted hydroxyapatite has good biocompatibility, and 10%,100% strontium substituted hydroxyapatite has weak cytotoxicity.
OBJECTIVE: To evaluate the cytotoxicity of strontium substituted hydroxyapatite. METHODS: Cell Relative Growth Rate(RGR) method, MTT assay and Flow Cytometry(FCM) method were used, and the strontium substituted hydroxyapatite contained different strontium concentration(0%,1%,5%,10%,100%). RESULTS: It was found that there's no apparent cytotoxicity of all the strontium substituted hydroxyapatite,but the cytotoxicity increased as the strontium concentration raised. As the FCM method appeared, there was no apparent difference between the pure hydroxyapatite and 1%,5% strontium substituted hydroxyapatite. CONCLUSION:Strontium substituted hydroxyapatite has good biocompatibility, and 10%,100% strontium substituted hydroxyapatite has weak cytotoxicity.