| Literature DB >> 14979433 |
Roberto Hamer-Barrera1, Delfino Godínez, V Idalia Enríquez, Sergio Vaca-Pacheco, Rodrigo Martinez-Zúñiga, Patricia Talamás-Rohana, Francisco Suárez-Güemez, Mireya de la Garza.
Abstract
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetra-acetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract.Entities:
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Year: 2004 PMID： 14979433 PMCID： PMC1142127
Source DB: PubMed Journal: Can J Vet Res ISSN： 0830-9000 Impact factor: 1.310