BACKGROUND: Based on previous pharmacokinetic and pharmacodynamic studies, we have developed an EEG-controlled closed-loop system for the i.v. hypnotic agent propofol in rats. METHODS: Seven adult male Sprague-Dawley rats (weight 423-584 g) were included in the study. EEG was recorded with occipito-occipital needle electrodes and the EEG power spectrum was estimated. The median frequency (MEF) was extracted from the power spectrum and was modified MEF (mMEF) to account for the occurrence of spikes and burst suppression patterns in the EEG. Propofol infusion was controlled by a model-based adaptive control algorithm to maintain a set point of mMEF=3.0 (sd 0.5) Hz. The performance of the feedback system was characterized by the median performance error MDPE=median[(mMEF-set point)/set point] and the median absolute performance error (MDAPE). The effective therapeutic infusion (ETI) to maintain the set point was determined from the resulting infusion rates. RESULTS: In all rats a feedback period of 90 min could be performed. Mean MDPE was 1.2 (se 0.4)% and MDAPE was 13.9 (0.3)%. The ETI was 0.73 (sd 0.20) mg kg(-1) min(-1). Mean arterial pressure before propofol infusion was 148 (14) mm Hg, with the lowest value during closed-loop infusion being 110 (20) mm Hg. CONCLUSIONS: The feedback system presented here may be a useful tool not only for automatic drug control to maintain a defined hypnotic effect but may also be a powerful device in pharmacological studies such as the determination of dose requirements or the assessment of drug-drug interactions.
BACKGROUND: Based on previous pharmacokinetic and pharmacodynamic studies, we have developed an EEG-controlled closed-loop system for the i.v. hypnotic agent propofol in rats. METHODS: Seven adult male Sprague-Dawley rats (weight 423-584 g) were included in the study. EEG was recorded with occipito-occipital needle electrodes and the EEG power spectrum was estimated. The median frequency (MEF) was extracted from the power spectrum and was modified MEF (mMEF) to account for the occurrence of spikes and burst suppression patterns in the EEG. Propofol infusion was controlled by a model-based adaptive control algorithm to maintain a set point of mMEF=3.0 (sd 0.5) Hz. The performance of the feedback system was characterized by the median performance error MDPE=median[(mMEF-set point)/set point] and the median absolute performance error (MDAPE). The effective therapeutic infusion (ETI) to maintain the set point was determined from the resulting infusion rates. RESULTS: In all rats a feedback period of 90 min could be performed. Mean MDPE was 1.2 (se 0.4)% and MDAPE was 13.9 (0.3)%. The ETI was 0.73 (sd 0.20) mg kg(-1) min(-1). Mean arterial pressure before propofol infusion was 148 (14) mm Hg, with the lowest value during closed-loop infusion being 110 (20) mm Hg. CONCLUSIONS: The feedback system presented here may be a useful tool not only for automatic drug control to maintain a defined hypnotic effect but may also be a powerful device in pharmacological studies such as the determination of dose requirements or the assessment of drug-drug interactions.
Authors: Joseph F Cotten; Ri Le Ge; Natalie Banacos; Ervin Pejo; S Shaukat Husain; James H Williams; Douglas E Raines Journal: Anesthesiology Date: 2011-10 Impact factor: 7.892
Authors: Nathalie Percie du Sert; Alessio Alfieri; Stuart M Allan; Hilary Vo Carswell; Graeme A Deuchar; Tracy D Farr; Paul Flecknell; Lindsay Gallagher; Claire L Gibson; Michael J Haley; Malcolm R Macleod; Barry W McColl; Christopher McCabe; Anna Morancho; Lawrence Df Moon; Michael J O'Neill; Isabel Pérez de Puig; Anna Planas; C Ian Ragan; Anna Rosell; Lisa A Roy; Kathryn O Ryder; Alba Simats; Emily S Sena; Brad A Sutherland; Mark D Tricklebank; Rebecca C Trueman; Lucy Whitfield; Raymond Wong; I Mhairi Macrae Journal: J Cereb Blood Flow Metab Date: 2017-08-11 Impact factor: 6.200
Authors: Daniel Kiefer; Lukas M Müller-Wirtz; Felix Maurer; Tobias Hüppe; Alexander M Mathes; Thomas Volk; Sascha Kreuer; Tobias Fink Journal: Exp Anim Date: 2021-12-08