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Literature DB >> 1497609

Uptake of Al3+ into the N-lobe of human serum transferrin.

G Kubal¹, A B Mason, P J Sadler, A Tucker, R C Woodworth.

Abstract

We have studied the binding of Al3+ to human serum apotransferrin (80 kDa) and recombinant N-lobe human apotransferrin (40 kDa) in 0.1 M-sodium bicarbonate solution at a pH meter reading in 2H2O (pH*) of 8.8 using 1H n.m.r. spectroscopy. The results show that for the intact protein, preferential binding of Al3+ to the N-lobe occurs. Molecular modelling combined with an analysis of ring-current-induced shifts suggest that n.m.r. spectroscopy can be used to probe hinge bending processes which accompany metal uptake in solution.

Entities: Chemical Gene Species

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Year: 1992 PMID： 1497609 PMCID： PMC1132852 DOI： 10.1042/bj2850711

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

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3 in total